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Luminita

Wednesday, 13 October 2010 21:48 Laboratories - Vascular Cell Proteomics
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CURRICULUM VITAE

IVAN (RĂDULESCU) G. LUMINIŢA

Date personale

Data şi locul naşterii: 5 martie 1970, Bucureşti, România

Sex: feminin

Starea civilă: căsătorită

Naţionalitatea: română

Locul de munca: Institutul de Biologie şi Patologie Celulară "Nicolae Simionescu", Str. B.P. Haşdeu, nr. 8, sect. 5, Bucureşti, 79691, România,

tel. (4021) 319 27 37/ 228

e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it ; This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Funcţia: Cercetător ştiinţific principal grad II


Studii Anul Institutia Specialitatea

Bacalaureat 1988 Liceul de Chimie Industrială Chimie

nr. 5, Bucuresti industrială

Licenţa 1994 Universitatea Bucuresti, Biochimie

Facultatea de Chimie

Doctorat 2004 Institutul de Biologie si Biologie celulara şi

Patologie Celulara moleculară

Post doctorat 2010 Institutul de Biochimie  Biotehnologii celulare si

moleculare cu aplicatii in medicina


Specializari

  • 1996 - cursurile Şcolii de vară francofone “Glycobiology and Glycotehnology”, Iaşi, România.
  • 1996 - bursă de specializare la Max - Planck Institute, Germania pentru învăţarea tehnicii de preparare a anticorpilor monoclonali.
  • 2002 - cursurile Atelierului de «Imunologie Fundamentală », organizat de Societate Nationala de Imunologie – Institutul National “Cantacuzino”.
  • 2004 - ECVAM Training Course in vitro Monoclonal Antibody Production, Netherland Vaccine Institute
  • 2005 - Course Proteomic- Bio Rad Laboratories, Budapesta, Ungaria
  • 2009 - Cell Culture Seminar by LGC Standards, Bucuresti
  • 2010 - Spectrometria de masa de inalta rezolutie: aplicatii in proteomica, Pro Analysis Systems, si Thermo Fisher Scientific, Bucuresti


Membră a unor societăţi ştiinţifice

1994 - prezent: Societatea Naţionala de Biologie Celulara;

1999 - prezent : ECBO (European Cell Biology Organization)

2004 - prezent: Societatea Nationala de Biochimie si Biologie Moleculara

Domeniul ştiinţific şi experienţa tehnică:

  • Culturi celulare.
  • Obţinerea de anticorpi policlonali si monoclonali.
  • Biologia celulara a endoteliului vascular: Transportul transendotelial al liganzilor.
  • Tehnici biochimice: dozări de proteine, electroforeze uni si bidimensionale, cromatografii, focalizare izoelectrica, autoradiografie,
  • Microscopie optică şi electronică, citochimie.
  • Radiomarcarea proteinelor, tehnici de spectrofotometrie si fluorescenţă.
  • Dozări radiobiochimice prin scintilaţie în lichid.

Limbi straine: engleza.


LUCRARI PUBLICATE IN REVISTE INTERNATIONALE COTATE ISI:

1. Felicia Antohe, Geo Serban, Luminita Radulescu, Maya Simionescu - Transcytosis of Albumin in Endothelial Cells is Brefeldin-A Independent, Endothelium, 5:125-136, 1997. (factor de impact 2,344; nr. citari ISI: 8)

2. Felicia Antohe, Doina Popov, Luminita Radulescu, Nicolae Simionescu, T. Börchers, F. Spener, Maya Simionescu - Endothelial cells from aorta and heart microvessels express the 15 kDa heart type FABP, European Journal of Cell Biology, vol. 76, 102-109, 1998. (factor de impact 3,039; nr. citari ISI: 9)

3. Felicia Antohe, Luminita Radulescu, Anca Gafencu, Victor Ghetie, Maya Simionescu Expression of Functionally Active FcRn and the Differentiated Bidirectional Transport of IgG in Human Placenta Endothelial Cells, Human Immunology, 62, 93-105, 2001. (factor de impact 2,619; nr. citari ISI:35)

4. Luminita Radulescu, Ileana Manduteanu, Felicia Antohe, Maya Simionescu- Detection of new antigens in activated endothelial cells by high-resolution 2-D gel technology, Electrophoresis - Tech Note 2979, Bio-Rad, 2003.

5. Ileana Manduteanu, Elena Dragomir, Felicia Antohe, Luminita Radulescu, Maya Simionescu, Effect of enoxaparin on high glucose induced activation of endothelial cells, Eur. J. Pharmacol, 477(3), 269-276, 2003. (factor de impact 2,522; nr. citari ISI: 10).

6. Luminita Radulescu, Camelia Stancu, Felicia Antohe – Antibodies against human oxidized low-density lipoprotein (LDL) as markers for human plasma modified lipoproteins, Medical Science Monitor, 10, 7, BR207-214, 2004. (factor de impact 1,607; nr. citari ISI: 4).

7. Luminita Radulescu, Felicia Antohe, Victor Jinga, Victor Ghetie, E. Sally Ward, Maya Simionescu - Neonatal Fc receptors discriminates and monitors the pathway of native and modified immunoglobulin G in human placental endothelial cells, Human Immunology, 65, 6, 578-585, 2004. (factor de impact 2,619; nr. citari ISI: 1)

8. Felicia Antohe, Luminita Radulescu, Elena Puchianu, Michael D. Kennedy, Philip Low, Maya Simionescu - Increased uptake of folate conjugates by activated macrophages in experimental hyperlipemia, Cell Tissue Res., 320(2):277-285, 2005. (factor de impact 2,991, nr. citari ISI: 5).

9. Antohe, F; Radulescu, L; Uyy, E, et al.- Folate receptors and proteomic analysis of endothelial cells in atherosclerosis, ATHEROSCLEROSIS SUPPLEMENTS Volume: 7 Issue: 3 Pages: 590-590 Published: JUN 2006. Factor de impact 5,875, Times Cited: 0.

10. Elena Uyy, Felicia Antohe, Luminita Ivan, Raluca Haraba, Dorel Lucian Radu, Maya Simionescu. Upregulation of caveolin-1 expression is associated with structural modifications of endothelial cells in diabetic lung, Microvascular Research, 79, 154-159, 2010. (FI 3.075)

11. Luminita Ivan, Felicia Antohe - Hyperlipidemia induces endothelial-derived foam cells in culture, Journal of Receptors and Signal Transduction, 30(2), 106-114, 2010.(FI 1.54)

Rezumate ale lucrarilor prezentae la manifestarile stiintifice internationale publicate in reviste de specialitate cu factor de impact ISI

1. Luminita Ivan, Felicia Antohe, Maya Simionescu, Expression of heat shock proteins in cholesterol-loaded endothelial-foam cells, Atherosclerosis, vol 9/1, ISSN 1567-5688, p 204, 2008. (FI: 5,875).

2. Felicia Antohe, Raluca Haraba, Luminita Ivan, Elena Uyy, Effect of fluvastatin sodium on the expression of caveolin-1 in normal and hyperlipidemic hamsters, Atherosclerosis, vol 9/1, ISSN 1567-5688, p 204, 2008. (FI:5,875).

3. Raluca Haraba, Luminita Ivan, Viorel Suica, Elena Uyy, Felicia Antohe - Protein and gene expression of High mobility group box 1 in hyperlipidemic models, Journal of Clinical Lipidology, vol. 2, no. 5S, p 83-86, 2008.

4. Elena Uyy, Luminita Ivan, Raluca Haraba, Felicia Antohe - Expression of caveolin-1 in hyperlipidemia: endothelial derived foam cells and hyperlipidemic hamsters; Journal of Clinical Lipidology, vol. 2, no. 5S, p 146-149, 2008.

LUCRARI PUBLICATE IN REVISTE NATIONALE RECUNOSCUTE CNCSIS:

1. Luminita Radulescu, Felicia Antohe - Preparation of Cationized Albumin as Probe for Cell Surface and Permeability Studies, Rev. Roum. Biochim, 34(1-4), 149-155, 1997.

2. Monica Raicu, Luminita Radulescu, Maya Simionescu - Clotrimazole inhibits human smooth muscle cell proliferation induced by angiotensin and bradykinin, Current Problems in Cellular and Molecular Biology, Eds. A. Ardelan, C. Craciun, vol. II, 158-162, 1997.

3. Luminita Radulescu, Felicia Antohe - Monoclonal Antibodies to the Synthetic Polypeptide PS-19, Current Problems in Cellular and Molecular Biology, 5, 511-516, 2001.

LUCRARI PREZENTATE LA MANIFESTARI STIINTIFICE INTERNATIONALE (ABSTRACT)

1. Luminita Radulescu, Felicia Antohe, Doina Popov, Torsten Borchers, Friedrich Spener, Maya Simionescu - Heart Microvessels and Aortic Endothelial Cells Express the 15 kDa Heart-Type Fatty Acid-Binding Proteins, The First International Meeting of the Romanian Society of Biochemistry and Molecular Biology, Rev. Roum. Bioch, 35, 1-2, Bucharest, Romania, 1998.

2. Luminita Radulescu, Emanuela Belici, Felicia Antohe, Victor Ghetie, E. S. Ward, Maya Simionescu – Internalization and transcytosis of IgG in human placental endothelial cells, ECBO, Bologna, Italy, abstract vol., 161, 1999.

3. Luminita Radulescu, Felicia Antohe, Anca Gafencu, Victor Ghetie, E. Sally Ward - Transcytosis of IgG in Endothelial Cells, First International Congress of the Romanian Society for Cell Biology, Abstract Book, vol. 28, p. 40, 2000.

4. F. Antohe, Luminita Radulescu, E.S. Ward, V. Ghetie, M. Simionescu - Transcytosis and Recirculation of IgG in Human Placenta Endothelial Cells, The XXIX Immunology Conference, Abstract Book pg. 82 (9), 2000.

5. Felicia Antohe, Luminita Radulescu, Victor Ghetie, Maya Simionescu- Differentiated Bi-directional Endocytosis and Transport of IgG in Human Placental Endothelial Cells, EURESCO Conference, Membrane Dynamics in Endocytosis, October Tomar, Portugal, 2000.

6. Luminita Radulescu, Felicia Antohe, Victor Ghetie, E. Sally Ward, Maya Simionescu- FcRn is Instrumental in Transcytosis in Human Placenta Endothelial Cells, 12th Balkan Biochemical Biophysical Days, abstract volume, 139, Bucharest, Romania, 2001.

7. F. Antohe, L. Radulescu, A. Gafencu, V. Ghe\ie, M. Simionescu. - Expression of functionally active FcRn and differentiated bidirectional transport of IgG in human placental endothelial cells. Poster, p. 73-74. A III-a Conferinta Internationala "Romania si Romanii in Stiinta Contemporana", Sinaia, Romania, iunie 2001.

8. L. Radulescu, I. Manduteanu, F. Antohe - A proteomic approach to identify antigens from normal and activated endothelial cells., Book of Abstract p: 43, Workshop “Cardiovascular Dysfunction in Hyperlipidemia and Diabetes, Jointly with Meeting of the COST ACTION B17 Working Group “Cardiovascular Dysfunctions”, 10-13 October, Bucharest, 2002

9. I. Manduteanu, E. Dragomir, M. Voinea, F. Antohe, M. Capraru, L. Radulescu. - Effect of heparins on high glucose induced activation of endothelial cells. Book of Abstract p: 30, Workshop “Cardiovascular Dysfunction in Hyperlipidemia and Diabetes, Jointly with Meeting of the COST ACTION B17 Working Group “Cardiovascular Dysfunctions”, 10-13 October, Bucharest, 2002.

10. F. Antohe, L. Radulescu, V. Ghetie, M. Simionescu - Differentiated Bidirectional Endocytosis and Transcytosis of IgG in Human Placental Endothelial Cells, Book of Abstract p: 55, Workshop “Cardiovascular Dysfunction in Hyperlipidemia and Diabetes, Jointly with Meeting of the COST ACTION B17 Working Group “Cardiovascular Dysfunctions”, 10-13 October, Bucharest, 2002.

11. I. Manduteanu, F. Antohe, E. Dragomir, M. Voinea, M. Capraru, L. Radulescu, M. Simionescu - Effect of enoxaparin on high glucose induced activation of endothelial cells, International Society of Thrombosis and Haemostasis, abstract volume, UK, 2003.

12. Luminita Radulescu, Felicia Antohe, Philip Low, Maya Simionescu - Endocytosis of folic acid by activated macrophages in experimental hyperlipemia, EURESCO Conferences: Membrane Dynalics in Endocytosis, Acquafredda di Maratea, Italy, 13-18 September 2003.

13. Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe, Maya Simionescu - Purification of caveolin-enriched membrane fraction from normal, diabetic and hyperlipidemic animals, Edinburgh, Scotland, UK, 29 Martie-8 Aprilie, 2004.

14. Luminita Radulescu, Felicia Antohe, Victor Ghetie, Maya Simionescu – FcRn discriminates and monitors the pathway of native and modified IgG in placental endothelial cells, Anniversary Workshop “Cell and molecular biology a key to defeat global risk diseases: atherosclerosis, diabetes and immune disorders”, Bucharest, Romania, 8-12 September, abstract volum, p 63, 2004.

15. Felicia Antohe, Luminita Radulescu, Elena Puchianu, Michael Kennedy, Philip Low, Maya Simionescu – Increased uptake of folate conjugates by activated macrophages in hyperlipemia, Anniversary Workshop “Cell and molecular biology a key to defeat global risk diseases: atherosclerosis, diabetes and immune disorders”, Bucharest, Romania, 8-12 September, abstract volum, p 45, 2004.

16. Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe – Over-expresion of caveolin-1 in diabetic and hyperlipidemic mice, Anniversary Workshop “Cell and molecular biology a key to defeat global risk diseases: atherosclerosis, diabetes and immune disorders”, Bucharest, Romania, 8-12 September, abstract volum, p 50, 2004.

17. Luminita Radulescu, Camelia Stancu, Felicia Antohe - Antibodies against human oxidized low-density lipoprotein (LDL) as markers for human plasma modified lipoproteins at “8 th International Conference Vascular Endothelium: Translating Discoveries into Public Health Practice”, Knossos Royal Village, Greece, June 25 – July 2 , abstract volume p 74, 2005.

18. Felicia Antohe, Luminita Radulescu, Elena Puchianu, Michael Kennedy, Philip Low, Maya Simionescu - Over-expression of folate receptors in activated macrophages in experimentsal hyperlipemia; a potential tool for drug targeting, at “8 th International Conference Vascular Endothelium: Translating Discoveries into Public Health Practice”, Knossos Royal Village, Greece, June 25 – July 2 , abstract volume p 59, 2005.

19. Felicia Antohe, Luminita Radulescu, Maya Simionescu - Proteomic analysis of endothelial cell-derived foam cells, HUPO, 28 august – 1 septembrie, Munich, Germania, 2005.

20. Luminita Radulescu, Camelia Stancu, Felicia Antohe - Antibodies against human oxidized low-density lipoprotein (LDL) as markers for human plasma modified lipoproteins at Simpozion “Combating cardiovascular disease and diabetes”, Bucuresti, 14 octombrie 2005.

21. Luminita Radulescu, Felicia Antohe, Maya Simionescu - Proteomic analysis of endothelial cell-derived foam cells, Simpozion “Combating cardiovascular disease and diabetes”, Bucuresti, 14 octombrie 2005.

22. Luminita Radulescu, Camelia Stancu, Felicia Antohe - Antibodies against human oxidized low-density lipoprotein (LDL) as markers for human plasma modified lipoproteins at Simpozion “New insights in molecular medicine”, Bucuresti, 4 noiembrie 2005.

23. Luminita Radulescu, Felicia Antohe, Maya Simionescu - Proteomic analysis of endothelial cell-derived foam cells, at Simpozion “New insights in molecular medicine”, Bucuresti, 4 noiembrie 2005.

24. Luminita Radulescu, Felicia Antohe, Maya Simionescu. Proteomic analysis of endothelial cell-derived foam cells for detection of modified peptides, Genomes to Systems Conference, Manchester, UK, 22-24 martie 2006.

25. Felicia Antohe, Luminita Radulescu, Elena Uyy, P.S. Low, M. Simionescu, Folate receptors and proteomic analysis of endothelial cells in atherosclerosis, XIV International Symposium on Atherosclerosis, Roma, Italia, 18-22 iunie 2006.

26. Radu Laura, Radulescu Luminita, Antohe Felicia, Simionescu Maya. Monoclonal antibodies as markers for human plasma modified lipoproteins. International One day Symposium, Iasi, 8 june 2006.

27. Radulescu Luminita, Radu Laura, Antohe Felicia. Expression of heat shock proteins in cholesterol-loaded endothelial-foam cells. International One day Symposium, Iasi, 8 june 2006.

28. Haraba Raluca, Uyy Elena, Radulescu Luminita, Antohe Felicia, Effect of human fluvastatin sodium on expression of Caveolin-1 in normal and hyperlipidemic hamsters. International One day Symposium, Iasi, 8 june 2006.

29. Felicia Antohe, Luminita Radulescu, Elena Uyy, P.S. Low, M. Simionescu, Folate receptors and proteomic analysis of endothelial cells in atherosclerosis, International One day Symposium, Iasi, 8 june 2006.

30. Luminita Radulescu, Laura Radu, Felicia Antohe, Maya Simionescu Proteomic analysis of endothelial cell-derived foam cells for detection of modified peptides. Workshop-ul inaugural al CDRTU: “Combaterea maladiilor cardiovasculare si a diabetului” 27-29 aprilie, Bucharest, Romania, 2006

31. Felicia Antohe, Luminita Radulescu, Elena Uyy, Philip Low, Maya Simionescu. Folate receptors and proteomic analysis of endothelial cells in atherosclerosis. Workshop-ul inaugural al CDRTU: “Combaterea maladiilor cardiovasculare si a diabetului” 27-29 aprilie, Bucharest, Romania, 2006.

32. Felicia Antohe, Luminita Radulescu, Raluca Haraba, Maya Simionescu - "Proteomic analysis of endothelial cell-derived foam cells", ESF ProteomeBinders Wokshop Affinity Proteomics, Alpbach, Austria 13-15 March 2007.

33. Luminita Ivan, Felicia Antohe - Endothelial-derived foam cells potential contributors to plaque vulnerability, International Symposium Translational Research in Vascular medicine, Bucuresti, 27-29 martie 2008.

34. Raluca Haraba, Luminiţa Ivan, Viorel Suica, Elena Uyy, Felicia Antohe - Expression HMGB1 protein in normal and hyperlipidemic hamster lungs, International Symposium Translational Research in Vascular Medicine, Bucuresti, 27-29 martie 2008.

35. Luminita Ivan, Felicia Antohe, Maya Simionescu - Expression of heat shock proteins in cholesterol- loaded endothelial- foam cells, “77th European Atherosclerosis Society Congress, Istanbul, Turkey, 26-29 April 2008.

36. Felicia Antohe, Raluca Haraba, Luminita Ivan, Elena Uyy – Effect of fluvastatin sodium on the expression of caveolin-1 in normal and hyperlipidemic hamsters, “77th European Atherosclerosis Society Congress, Istanbul, Turkey, 26-29 April 2008.

37. Elena Uyy, Luminita Ivan, Raluca Haraba, Viorel Şuică, Felicia Antohe- Effect of folic acid deficient diet and fluvastatin sodium on the expression of caveolin 1 in normal and hyperlipidemic hamsters, Workshop Exploratoriu “De la descoperirile cercetării fundamentale la aplicaţii terapeutice”, Bucureşti, 17 – 18 septembrie 2008.

38. Luminiţa Ivan, Felicia Antohe, Maya Simionescu - Endothelial cell-derived foam cell potential candidate responsible for plaque vulnerability- Workshop Exploratoriu “De la descoperirile cercetării fundamentale la aplicaţii terapeutice” Bucureşti, 17 – 18 septembrie 2008 .

39. Raluca Haraba, Luminiţa Ivan, Viorel Suica, Elena Uyy, Felicia Antohe - Protein expression of high mobility group box 1 in hyperlipidemic hamsters, Workshop Exploratoriu “De la descoperirile cercetării fundamentale la aplicaţii terapeutice” Bucureşti, 17 – 18 septembrie 2008.

40. Viorel Suică, Luminţa Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe - Proteomica diferenţială a rafturilor lipidice în condiţii normale şi hiperlipidemice, Workshop Exploratoriu “De la descoperirile cercetării fundamentale la aplicaţii terapeutice” Bucureşti, 17 – 18 septembrie 2008.

41. Luminita Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe - Expression of heat shock proteins in lipid loaded endothelial-derived foam cells, 4th International CONFERENCE “The Hsp90 Chaperone Machine” organizata de J. Buchner si D. Picard in Seeon, Germany, 2-6 octomber 2008.

42. Raluca Haraba, Luminita Ivan, Viorel Suica, Elena Uyy, Felicia Antohe - Protein and gene expression of high mobility group box 1 in hyperlipidemic models - 7th International Symposium “Multiple risk factors in cardiovascular diseases prevention and intervention- Health Policy” Venice (Lido), Italy, 22-25 octomber 2008.

43. Elena Uyy, Luminita Ivan, Raluca Haraba, Felicia Antohe – Expression of caveolin 1 in hyperlipidemia: endothelial derived foam cells and hyperlipidemic hamsters, 7th International Symposium “Multiple risk factors in cardiovascular diseases prevention and intervention - Health Policy” Venice (Lido), Italy, 22-25 octomber, 2008.

44. Raluca Haraba, Luminita Ivan, Viorel Suica, Elena Uyy, Felicia Antohe, Maya Simionescu - Expression of high mobility group box 1 protein in Hyperlipidemic models, ICBP 30 years Anniversary Simposium, Bucharest, Romania, 10-12 septembrie 2009.

45. Luminita Ivan, Elena Uyy, Raluca Haraba, Viorel Suica, Felicia Antohe - Expression of heat shok proteins in lipid loaded endothelial-derived foam cells, ICBP 30 years Anniversary Simposium, Bucharest, Romania, 10-12 septembrie 2009.

46. Elena Uyy, Luminita Ivan, Raluca Haraba, Radu Dorel, Felicia Antohe, Maya Simionescu - Upregulation of caveolin-1 expression in pulmonary caveolin-rich membrane domains in diabetes, ICBP 30 years Anniversary Simposium Bucharest, Romania, 10-12 septembrie 2009.

47. Raluca Haraba, Luminita Ivan, Elena Uyy, Viorel Suica, Felicia Antohe, The efect of fluvastatin treatment on HMGB1 in hyperlipidemic animal model, 8th International Congress on Coronary Artery Disease, Prague, Czech Republic, Octombrie 11-14, 2009.

48. Luminita Ivan, Elena Uyy, Raluca Haraba, Viorel Suica, Felicia Antohe, Maya Simionescu, Hyperlipidemia induces endothelial-derived foam cells formation, 8th International Congress on Coronary Artery Disease,Prague, Czech Republic, Octombrie 11-14, 2009.

49. Viorel Suică, Luminita Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe, Proteomic approach of plasma membrane microdomains in experimental hyperlipidemia - 4th Central and Eastern European Proteomics Conference, Viena , Austria, 29.08.2010-1.10. 2010.

LUCRARI PREZENTATE LA MANIFESTARI STIINTIFICE NATIONALE

(ABSTRACT)

1. Luminita Radulescu, Felicia Antohe - Preparation of Cationized Albumin as Probe for Cell Surface and Permeability Studies, The Bulletin of the National Society of Cellular Biology, 23, p. 62,1995.

2. Luminita Radulescu, Felicia Antohe, Maya Simionescu - The Production of Hibridomas Secreting Monoclonal Antibody to Human Recombined Annexin V, The Bulletin of the National Society of Cellular Biology, 24, p. 79, 1996.

3. Luminita Radulescu, Felicia Antohe, Maya Simionescu - The Production of Monoclonal Antibody to PS-19, The Bulletin of the National Society of Cellular Biology, Abstract volume, 25, 74, 1997.

4. Felicia Antohe, Olga Starodub, Carmen Cristea, Luminita Radulescu, Victor Ghetie, Maya Simionescu - The intracellular distribution of FcRn receptor in endothelial cells, The Bulletin of the National Society of Cellular Biology, Abstract volume, 25, 71, 1997.

5. Felicia Antohe, Luminita Radulescu, Emanuela Belici, E. Sally Ward, Victor Ghetie, Maya Simionescu - The expression of FcRn receptor in endothelial cells, The Bulletin of the National Society of Cellular Biology, Abstract volume, 26, 108, 1998.

6. Luminita Radulescu, Emanuela Belici, Felicia Antohe, Victor Ghetie, E. Sally Ward, Maya Simionescu– Internalization and transcytosis of IgG in human placental endothelial cells, The Bulletin of the National Society of Cellular Biology, Abstract Book, 27, 76, 1999.

7. Luminita Radulescu, Felicia Antohe, Camelia Stancu, Anca Sima - The Production of Monoclonal Antibody to LDL oxidation, The Bulletin of the National Society of Cellular Biology, abstract volume, 29, 2001.

8. Luminita Radulescu, Felicia Antohe, Camelia Stancu, Anca Sima - The Production and the Purification of Monoclonal Antibody to LDL oxidation, The Bulletin of the National Society of Cellular Biology, abstract volume, 30, 2002.

9. F. Antohe, L. Radulescu, V. Ghetie - Role of FcRn receptor in transfer materno- fetal of IgG, The Bulletin of the National Society of Cellular Biology, abstract volume, 30, 2002.

10. Manduteanu, F. Antohe, E. Dragomir, M. Voinea, Luminita Radulescu, M. Capraru, M. Simionescu - Effect of enoxaparin on high glucose induced activation of endothelial cells, The Bulletin of the National Society of Cellular Biology, abstract volume, 30, 2002.

11. Luminita Radulescu, S. Hristescu, D. Troznay, G. Ilie, D.L. Radu, F. Antohe, A. Onu- Rezultatele obtinute in cadrul proiectului “ Stabilirea unei tehnologii de producere a anticorpilor monoclonali prin crearea unui centru national de productie de anticorpi monoclonali”, lucrare in cadrul Simpozionului “ Prioritati in dezvolatrea Biotehnologiei romanesti’, Tirgoviste, 12-13 decembrie 2002.

12. Luminita Radulescu, Elena Puchianu, Ileana Manduteanu, Felicia Antohe - Analiza proteomica a celulelor endoteliale activate cu seruri umane hiperglicemice si hipercolesterolemice, The Bulletin of the National Society of Cellular Biology, abstract volume 31, 2003.

13. Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe - Prepararea unei fractii membranare imbogatita in caveolina, The Bulletin of the National Society of Cellular Biology, abstract volume 31, 2003.

14. Felicia Antohe, Luminita Radulescu, Philip Low, Maya Simionescu - Expresia receptorilor la acid folic pe macrofagele activate in conditii de hipercolesterolemie, The Bulletin of the National Society of Cellular Biology, abstract volume 31, p. 66, 2003.

15. Madalina Cojocaru, Coziana Ciurtin, Irina Maria Miron, Luminita Radulescu, Felicia Antohe – Medicatia biologica in terapia de fond a artritei reumatoide, The Bulletin of the National Society of Cellular Biology, abstract volume 31, p. 62, 2003.

16. Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe - Expresia caveolinei-1 in fractii membranare pulmonare in conditii normale si patologice, The Bulletin of National Society of Cellular Biology, abstract volume 32, 2004.

17. Felicia Antohe, Luminita Radulescu, Elena Puchianu, Philip Low, Maya Simionescu- Increased uptake of folate conjugates by activated macrophages in experimental hyperlipemia, The Bulletin of National Society of Cellular Biology, abstract, volume 32, 2004.

18. Luminita Radulescu, Victor Ghetie, Felicia Antohe- Neonatal Fc receptors (FcRn) discriminates and monitors the pathway of native and modified immunoglobulin G in placental endothelial cells, The Bulletin of National Society of Cellular Biology, abstract, volume 32, 2004.

19. Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe, Maya Simionescu - Imunodetectia caveolinei-1 in fractii membranare pulmonare in soareci cu diabet si hiperlipidemie, A 34-a Conferinta Nationala de Imunologie, Bucuresti, 16-17 septembrie, abstract volume 30, 2004.

20. Luminita Radulescu, Felicia Antohe - Model experimental de obtinere a celulelor endoteliale spumoase in cultura. A XXIII-a Sesiune Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Sibiu, 9-12 iunie, Buletin SNBC, nr. 33, p.248, 2005.

21. Felicia Antohe, Luminita Radulescu, Elena Puchianu, Philip S Low, Maya Simionescu - Macrofagele activate in conditii de hipercolesterolemie exprima receptori pentru acid folic. A XXIII-a Sesiune Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Sibiu, 9-12 iunie, Buletin SNBC, nr. 33, p.78, 2005.

22. Elena Puchianu, Luminita Radulescu, Felicia Antohe - Imunodetectia caveolinei 1 in fractii membranare pulmonare de la hamsteri cu ateroscleroza si deficit de acid folic. A XXIII-a Sesiune Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Sibiu, 9-12 iunie, Buletin SNBC, nr. 33, p.147, 2005.

23. Luminita Ivan, Elena Uyy, Felicia Antohe - Expresia proteinelor de soc termic (HSP) in celulele endoteliale incarcate cu lipide. Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Cluj, 9-12 iunie, Buletin SNBC, nr. 36, pag 45, 2008.

24. Elena Uyy, Luminita Ivan, Raluca Haraba, Felicia Antohe – Imunodetectia caveolinei-1 in celulele endoteliale incarate cu lipide si in hamster hiperlipidemic, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Cluj, 9-12 iunie, Buletin SNBC, nr. 36, 2008.

25. Viorel Suica, Luminita Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe –Proteomica diferentiala a rafturilor lipidice in conditii normale si hiperlipidemice, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Cluj, 9-12 iunie, Buletin SNBC, nr. 36, 2008.

26. Raluca Haraba, Luminiţa Ivan, Viorel Suica, Elena Uyy, Felicia Antohe - Expresia proteinei nucleare HMGB1 in hamsteri normali si hiperlipidemici, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Cluj, 9-12 iunie, Buletin SNBC, nr. 36, pag. 47, 2008.

27. Viorel Suica, Luminita Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe - Proteomica diferentiala a rafturilor lipidice in conditii normale si hiperlipidemice, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Bistrita, 9-12 iunie, Buletin SNBC, nr. 37, pag 72, 2009.

28. Luminita Ivan, Elena Uyy, Raluca Haraba, Viorel Suica, Felicia Antohe- Expresia proteinelor de soc termic (HSP) in conditii de hiperlipidemie, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Constanta, 9-12 iunie, Buletin SNBC, nr. 38, pag 234, 2010.

29. Elena Uyy, Felicia Antohe, Luminita Ivan, Raluca Haraba, Dorel L. Radu, Maya Simionescu – Expresia caveolinei-1 in fractii membranarepulmonare din soareci transgenici cu diabet zaharat de tip I, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Constanta, 9-12 iunie, Buletin SNBC, nr. 38, pag 116, 2010.

30. Raluca Haraba, Viorel Suică, Elena Uyy, Luminita Ivan, Felicia Antohe - Caile de semnalizare induse de proteina nucleara HMGB1 in conditii hiperlipidemice, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Constanta, 9-12 iunie, Buletin SNBC, nr. 38, pag 142, 2010.

PREMII:

1. Premiul BIO-RAD Laboratories pentru rezultate deosebite obtinute in activitatea de cercetare cu echipamentele BIO-RAD Protean IEF si Protean II XI pentru lucrarea: A proteomic approach to identify antigens from normal and activated endothelial cells - Luminita Radulescu, Ileana Manduteanu, Felicia Antohe, Book of Abstract p: 43, Workshop “Cardiovascular Dysfunction in Hyperlipidemia and Diabetes, Jointly with Meeting of the COST ACTION B17 Working Group “Cardiovascular Dysfunctions”, 10-13 October 2002, Bucharest.

2. EURESCO Conference, Membrane Dynamics in Endocytosis, October 2001, Tomar, Portugal. Their poster entitled “ Differentiated Bi-directional Endocytosis and Transport of IgG in Human Placental Endothelial Cells”, Felicia Antohe, Luminita Radulescu, Victor Ghetie and Maya Simionescu was awarded with the EURESCO prize.

3. Premiul “Ion Moraru” de Imunologie Fundamentala – Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe, “Imunodetectia caveolinei-1 in fractii membranare pulmonare in soareci cu diabet si hiperlipidemie”, A 34-a Conferinta Nationala de Imunologie, – 16-17 Septembrie, Bucuresti, Romania, abstract volume 34, 30-31: 2004 ( comunicare in plen).

4. Luminita Radulescu, Camelia Stancu, Felicia Antohe: The Best Poster of the Conference: “8th International Conference Vascular Endothelium: Translating Discoveries into Public Health Practice”, Knossos Royal Village, Greece, June 25 – July 2, 2005

5. Luminita Radulescu for successful scientific activities in the sixth framework program of the European Community, 2006

6. Premiul III cu posterul: Viorel Suica, Luminita Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe - PROTEOMICA DIFERENTIALA A RAFTURILOR LIPIDICE IN CONDITII NORMALE SI HIPERLIPIDEMICE, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Bistrita, 9-12 iunie, Buletin SNBC, nr. 37, pag 72, 2009.

7. Premiul III cu posterul: Raluca Haraba, Viorel Suică, Elena Uyy, Luminita Ivan, Felicia Antohe - Caile de semnalizare induse de proteina nucleara HMGB1 in conditii hiperlipidemice, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Constanta, 9-12 iunie, Buletin SNBC, nr. 38, pag 142, 2010

ALTE Prezentari STIINTIFICE

  1. 2001 - Prezentare in cadrul lectiei: Culturile celulare – alternativa la modelele experimentale animale. Tehnologie, aplicatii.
  2. F. Antohe, L.Radulescu , V. Ghetie, S. E. Ward, M. Simionescu- Rolul receptorilor FcRn in celulele endoteliale, prezentare in cadrul Cursului post- universitar de perfectionare: “De la biologia celulara si moleculara la medicina secolului XXI”, 28mai- 5 iunie 2001, organizat de Academia Romana si IBPC “N.Simionescu”.
  3. Prezentare in cadrul Cursului post-universitar de perfectionare: “De la biologia celulara si moleculara la medicina secolului XXI”, 10-18 octombrie 2002, organizat de Academia Romana si IBPC “N.Simionescu”: Culturile celulare – alternativa la modelele experimentale animale. Tehnologia producerii de anticorpi monoclonali.
  4. F. Antohe, L. Radulescu, V. Ghetie, S. E. Ward, M. Simionescu- Endothelial Cells the Site of the Regulation of IgG Level by Salvaging and Transcytosis of Immunoglobulin, conferinta sustinuta la Institutul de Biochimie al Academiei Romane, 2002.
  5. Participarea la “ Cursul postuniversitar de perfectionare - De la biologia Celulara si Moleculara la Medicina Secolului XXI ‘’ , IBPC, iunie 2003. Luminita Radulescu - Tehnologia producerii de anticorpi monoclonali. Culturile celulare- alternativa la modelele experimentale animale.
  6. Participarea la “ Cursul postuniversitar de perfectionare - De la biologia Celulara si Moleculara la Medicina Secolului XXI ‘’ , IBPC, iunie 2003- Luminita Radulescu - Tehnica de electroforeza bidimensionala, etapa a analizei proteomice.
  7. Luminita Radulescu, Felicia Antohe, Maya Simionescu - Proteomic analysis of endothelial cell-derived foam cells, Anniversary Workshop “Cell and molecular biology a key to defeat global risk diseases: atherosclerosis, diabetes and immune disorders”, Bucharest, Romania, 8-12 September, 2004.

8. Luminita Radulescu – curs: Tehnologia producerii de anticorpi monoclonali; Curs Postuniversitar de perfectionare:”De la biologia celulara si moleculara la medicina secolului XXI”, 14 -21 octombrie 2005.

9. Luminita Radulescu – curs: Demonstratie practica; tehnica de electroforeza bidimensionala; Curs Postuniversitar de perfectionare:”De la biologia celulara si moleculara la medicina secolului XXI”, 14 -21 octombrie 2005.

10. Viorel Suica, Elena Uyy, Raluca Haraba, Luminita Radulescu, Felicia Antohe, Lipid raft proteomics in hyperlipidemic conditions, Symposium of the COST Action BM0602 WG3 and WG4 Meeting, 13-14 February 2009.

11. Viorel Suica, Elena Uyy, Raluca Haraba, Luminita Radulescu, Felicia Antohe, High resolution proteomic analysis of plasma membrane microdomains, Proteomics Roadshow – BioRad Laboratories, 24 aprilie 2009.

12. Viorel Suica, Elena Uyy, Raluca Haraba, Luminita Radulescu, Felicia Antohe, High resolution proteomic analysis of hyperlipidemic plasma membrane microdomain”, ICBP „N. Simionescu“30 years Anniversary Simposium, Bucharest, Romania, 10-12 septembrie 2009.

13. Luminita Ivan, Elena Uyy, Raluca Haraba, Viorel Suica, Felicia Antohe Proteomic analysis of lipid loaded endothelial-derived foam cells, Al 6-lea Simpozion National de patologie “Patologie celulara si moleculara”, Sesiune Anuala a Institutului National “Victor Babes”, 3-5 noiembrie 2009.

Referent invitat pentru articolele:

1. „Cellular Machinery for Protein Synthesis is Highly Upregulated in VEGF-Activated Human Endothelial         Cells” by Czeslaw Cierniewski publicat in Proteomics, 2004.
  1. "Thrombin and Histamine Stimulate Endothelial Nitric-oxide Synthase Phosphorylation at Ser1177 via a PI3K-Akt-independent, AMPkinase-dependent Pathway" by Brynhildur Thors, Haraldur Halldórsson, Gudmundur Thorgeirsson din FEBS Letters, 2004.
  2. "High Sensitivity in Detection and Screening of Disease Biomarkers using Protein Lysate Microarrays" by Anitha Ramaswamy, E Lin, Kevin Coombes, Iou Chen, Joel Morrisett, Rahul Mitra, Mini Kapoor, din Proteomics, 2004.
4. “Hepatocyte Steatosis Increases the Expression of Adhesion Molecules in Endothelial Cell” by Gang Lin,
Xiaoyan Duan, Xiaobo Cai, Liyan Tian, Zhengjie Xu Jiangao Fan din Saudi Medical Journal, 2010.

LISTA CONTRACTELOR EXTRABUGETARE

Colaborator la urmatoarele proiecte:

1. - 1996 – 1998 - Grant from Swiss National Science Foundation: Transport pathways of IgG in placental endothelial cells, colaborare cu Walter Hunziker.

2. - 1997-1999 - Grant with the University of Texas, Dallas: Transport and Catabolism of IgG in Endothelial Cells, colaborare cu Victor Ghetie.

3. - 1997- Academia Romană - Identificarea şi localizarea proteinelor transportoare de acizi graşi în celulele endoteliale – Felicia Antohe, Luminiţa Rădulescu

4. - 1997 – M.C.T. Orizont – Studiul distribuţiei receptorului la LDL pe suprafaţa bazală şi apicală a celulelor endoteliale în cultură – Felicia Antohe, Luminiţa Rădulescu .

5. - 1997 – CNCSU – Detecţia în celulele endoteliale a proteinelor care leagă specific acizii graşi – Felicia Antohe, Luminiţa Rădulescu .

6. - 1998 - Academia Romana - Studii asupra receptorului pentru lipoproteine din membrana celulei endoteliale: implicaţii biologice şi funcţionale - Felicia Antohe, Luminiţa Rădulescu.

7. - 1998 - M.C.T. - Preluarea LDL şi a liposomilor de către neutrofile şi celulele endoteliale crescute pe filtre semipermeabile - Felicia Antohe, Luminiţa Rădulescu.

8. - 1998-1999 - MCT Orizont – Studii asupra receptorului pentru lipoproteine din membrana celulei endoteliale: implicatii biologice si functionale.

9. - 1999 - GAR–MCT: Implicarea celulelor endoteliale si a trofoblastelor in transportul directionat al IgG.

10 - 1999 - 2001 - ANSTI- Stiinte Biologice - Rolul receptorilor la IgG in celulele endoteliale placentare umane.

11- 1999 - 2000 - CNCSU – Producerea si selectionarea hibridoamelor care produc anticorpi monoclonali la peptidul sintetic PS-19 – responsabil de grant: Luminita Radulescu

12 - 1999- 2001 - Grant ANSTI, Stiinte Biologice- Rolul receptorilor la IgG in celulele endoteliale placentare umane. A. Gafencu, C. Heltianu, A. Burlacu, F. Antohe, L. Radulescu, V. Jinga, M. Simionescu

13 - 2001- 2002 - Grant MEC: Rolul receptorilor FcRn in asigurarea imunitatii pasive si in mentinerea homeostaziei IgG

14- 2001 - 2003 CERES: Rolul veziculelor plasmalemale (caveole) in mecanismele de transport al macromoleculelor prin endoteliul vascular, Felicia Antohe, Luminita Radulescu

15- 2001 - 2002 - BIOTECH: Stabilirea unei tehnologii de obtinere a anticorpilor monoclonali prin crearea unui centru national de productie a anticorpilor monoclonali, Responsabil de proiect: Luminita Radulescu

16- 2001 - 2002 GAR - Stiinte Medicale - Rolul receptorilor FcRn in transferul maternofetal al imunoglobulinei G.

17 - 2003 - GAR, Stiinte biologice - Transcitoza immunoglobulinei G prin celulele endoteliale placentare umane – Felicia Antohe, Luminita Radulescu, Maya Simionescu                                              

18 - 2003 – 2004 - CERES - Studii asupra vasculaturii cerebrale in procesul de imbatrinire, Elena Constantinescu, Felicia Antohe, Luminita Radulescu

19 - 2004 - 2006 - GAR - Expresia caveolinei 1 in fractii membranare pulmonare in conditii normale si patologice, director de proiect : dr. Felicia Antohe, Luminita Radulescu, 85 milioane lei

20 - 2004 - 2005 CNCSIS - Analiza biochimica si imunologica a fluidelor oculare ca metoda de diagnostic precoce al afectarii analizorului vizual in boli metabolice si reumatismale, director de proiect : dr. Viorica Madalina Cojocaru, Luminita Radulescu, 95 milioane lei.

21 - 2004 – 2006 - VIASAN - Analiza proteomica comparativa a celulelor endoteliale normale si “activate” prin expunere la ser uman hiperlipidemic si hiperglicemic, director de proiect: acad. Maya Simionescu, Felicia Antohe, Luminita Radulescu, 1,2 miliarde lei.

22 - 2005 - 2008 - Grant CEEX colaborare: AMCADO IBPC N.S: Alterarea mecanismelor celulare si moleculare si a expresiei genice in boli cardiovasculare si diabet/obezitate, afectiuni majore ale sindromului metabolic - cercetari fundamentale si clinice, 15 miliarde.

23 - 2005 - 2007 - Grant CEEX colaborare: Cercetari avansate in toleranta imuna cu scopul identificarii de cai terapeutice novatoare in patologia bolilor autoimune de tesut vasculo-conjunctiv. 1 miliard.

24 - 2005 - 2008 - Grant CEEX colaborare: Biotehnologii avansate de preparare a unor produse biologice terapeutice de uz uman şi de diagnostic cu integrarea unor metode optimizate de asigurare a calitătii şi de control, în conformitate cu standardele şi normele europene. 500 milioane.

25 -2007- 2010 - PN-II-ID-PCE-2007 colaborare: Mecanisme celulare si moleculare implicate in dezvoltarea placii aterosclerotice.

Data: Semnatura:

24.08. 2010


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