CURRICULUM VITAE
UYY (PUCHIANU) ELENA
Date personale
Data şi locul naşterii:19 noiembrie 1974, Bucureşti, România
Starea civilă: căsătorită
Naţionalitatea: română
Locul de munca: Institutul de Biologie şi Patologie Celulară "Nicolae Simionescu", Str. B.P. Haşdeu, nr. 8, sect. 5, Bucureşti, 79691, România, tel. (4021) 319 27 37
e-mail:
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Studii:
1990-1994 - Liceul Sanitar Bucuresti - Sora medicala
1995-1996 - Scoala Postliceala Sanitara Bucuresti - Asistent Medical Generalist
1997-2001- Universitatea Bucuresti, Facultatea de Fizica- Licentiat in Fizica
2001-2003 Universitatea Bucuresti, Facultatea de Fizica - Master in Biofizica
2003-prezent - Institutul de Biologie si Patologie Celulara “N. Simionescu” - Doctorand
Funcţia: Cercetător ştiinţific
Experienta profesionala:
1995-1997 - asistent medical – Sectia de Hemodializa, Spitalul Clinic “Sf.Ioan”, Bucuresti
2001-2002 - profesor de fizica – MECT, Bucuresti
2002-2004 - Asistent de cercetare - IBPC “N. Simionescu”,Bucuresti
2004-prezent - Cercetator stiintific - IBPC “N. Simionescu”,Bucuresti
Specializari
2003 - Cursul Postuniversitar de perfectionare- „De la Biologia Celulara si Moleculara la Medicina Secolului XXI”, organizat de Institutul de Biologie si Patologie Celulara “N. Simionescu”, Bucuresti, Romania, iunie 2003
2004 - Scoala de vara -Soft Condensed Matter Physics in Molecular and Cell Biology, organizata de NATO Advanced Study Institute, Edinburgh, Scotland, UK, 29 Martie-8 Aprilie,
2006 – Immunophysiology of infections may 2nd-12th Course organized by Pasteur Network and National Institute of Research and Development for Microbiology and Immunology “Cantacuzino,”
2010 - Spectrometria de masa de inalta rezolutie: aplicatii in proteomica, Pro Analysis Systems, si Thermo Fisher Scientific, Bucuresti
Membră a unor societăţi ştiinţifice
2003 - prezent: Societatea Romana de Biologie Celulara;
Domeniul ştiinţific şi experienţa tehnică:
· Culturi celulare.
· Biologia celulara a endoteliului vascular:
· Tehnici biochimice: dozări de proteine, electroforeze uni si bidimensionale, autoradiografie,
· Microscopie optică şi electronică, citochimie.
· Tehnici de spectrofotometrie si fluorescenţă.
Limbi straine: engleza, franceza
LUCRARI PUBLICATE IN REVISTE INTERNATIONALE COTATE ISI:
1. Felicia Antohe, Luminita Radulescu, Elena Puchianu, Michael D. Kennedy, Philip Low, Maya Simionescu - Increased uptake of folate conjugates by activated macrophages in experimental hyperlipemia, Cell Tissue Res., 320(2):277-285, 2005. (factor de impact 2,991, nr. citari ISI: 5).
2. Antohe, F; Radulescu, L; Uyy, E, et al.- Folate receptors and proteomic analysis of endothelial cells in atherosclerosis, Atherosclerosis Suplements, Volume: 7, Issue: 3 Pages: 590-590 Published: Jun 2006. (Factor de impact 5,875).
3. Elena Uyy, Felicia Antohe, Luminita Ivan, Raluca Haraba, Dorel Lucian Radu, Maya Simionescu - Upregulation of caveolin-1 expression is associated with structural modifications of endothelial cells in diabetic lung, Microvascular Research, 79, 154-159, 2010. (FI 3.075)
Rezumate ale lucrarilor prezentate la manifestarile stiintifice internationale publicate in reviste de specialitate cu factor de impact ISI
1. Felicia Antohe, Raluca Haraba, Luminita Ivan, Elena Uyy, Effect of fluvastatin sodium on the expression of caveolin-1 in normal and hyperlipidemic hamsters, Atherosclerosis, vol 9/1, ISSN 1567-5688, p 204, 2008. (FI:5,875).
2. Raluca Haraba, Luminita Ivan, Viorel Suica, Elena Uyy, Felicia Antohe - Protein and gene expression of High mobility group box 1 in hyperlipidemic models, Journal of Clinical Lipidology, vol. 2, no. 5S, p 83-86, 2008.
3. Elena Uyy, Luminita Ivan, Raluca Haraba, Felicia Antohe - Expression of caveolin-1 in hyperlipidemia: endothelial derived foam cells and hyperlipidemic hamsters; Journal of Clinical Lipidology, vol. 2, no. 5S, p 146-149, 2008.
LUCRARI PUBLICATE IN REVISTE NATIONALE RECUNOSCUTE CNCSIS:
Cojocaru, V MCiurtin, CPuchianu, EAntohe, FLacrimal proteins electrophoretic analysis--diagnostic method in secondary Sjogren syndrome, Journal: Oftalmologia (Bucharest, Romania : 1990), Vol. 50 (4): 60-63, 2006
2. Cojocaru, V MCiurtin, CPuchianu, EAnothe, FElectrophoretic analysis of lacrimal proteins-a diagnostic method in secondary Sjogren syndrome, Journal: Oftalmologia (Bucharest, Romania : 1990), Vol. 50 (3): 3-5, 2006
LUCRARI PREZENTATE LA MANIFESTARI STIINTIFICE INTERNATIONALE (ABSTRACT)
1. Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe, Maya Simionescu - Purification of caveolin-enriched membrane fraction from normal, diabetic and hyperlipidemic animals, Edinburgh, Scotland, UK, 29 Martie-8 Aprilie, 2004.
2. V.M.Cojocaru, E. Puchianu, F.Antohe, C. Ciurtin - The diagnosis of secondary Sjogren syndrome using tears proteins electrophoresis, poster – 11th International Student Congress of Medical Sciences, Groningen, Olanda, iunie 2004
3. Felicia Antohe, Luminita Radulescu, Elena Puchianu, Michael Kennedy, Philip Low, Maya Simionescu – Increased uptake of folate conjugates by activated macrophages in hyperlipemia, Anniversary Workshop “Cell and molecular biology a key to defeat global risk diseases: atherosclerosis, diabetes and immune disorders”, Bucharest, Romania, 8-12 September, abstract volum, p 45, 2004.
4. Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe – Over-expresion of caveolin-1 in diabetic and hyperlipidemic mice, Anniversary Workshop “Cell and molecular biology a key to defeat global risk diseases: atherosclerosis, diabetes and immune disorders”, Bucharest, Romania, 8-12 September, abstract volum, p 50, 2004.
5. Felicia Antohe, Luminita Radulescu, Elena Puchianu, Michael Kennedy, Philip Low, Maya Simionescu - Over-expression of folate receptors in activated macrophages in experimentsal hyperlipemia; a potential tool for drug targeting, at “8 th International Conference Vascular Endothelium: Translating Discoveries into Public Health Practice”, Knossos Royal Village, Greece, June 25 – July 2 , abstract volume p 59, 2005.
6. Felicia Antohe, Luminita Radulescu, Elena Uyy, P.S. Low, M. Simionescu, Folate receptors and proteomic analysis of endothelial cells in atherosclerosis, XIV International Symposium on Atherosclerosis, Roma, Italia, 18-22 iunie 2006.
7. Haraba Raluca, Uyy Elena, Radulescu Luminita, Antohe Felicia, Effect of human fluvastatin sodium on expression of Caveolin-1 in normal and hyperlipidemic hamsters. International One day Symposium,
8. Felicia Antohe, Luminita Radulescu, Elena Uyy, P.S. Low, M. Simionescu, Folate receptors and proteomic analysis of endothelial cells in atherosclerosis, International One day Symposium, Iasi, 8 june 2006.
9. Felicia Antohe, Luminita Radulescu, Elena Uyy, Philip Low, Maya Simionescu. Folate receptors and proteomic analysis of endothelial cells in atherosclerosis. Workshop-ul inaugural al CDRTU: “Combaterea maladiilor cardiovasculare si a diabetului” 27-29 aprilie, Bucharest, Romania, 2006.
10. Raluca Haraba, Luminiţa Ivan, Viorel Suica, Elena Uyy, Felicia Antohe - Expression HMGB1 protein in normal and hyperlipidemic hamster lungs, International Symposium Translational Research in Vascular Medicine, Bucuresti, 27-29 martie 2008.
11. Felicia Antohe, Raluca Haraba, Luminita Ivan, Elena Uyy – Effect of fluvastatin sodium on the expression of caveolin-1 in normal and hyperlipidemic hamsters, “77th European Atherosclerosis Society Congress, Istanbul, Turkey, 26-29 April 2008.
12. Elena Uyy, Luminita Ivan, Raluca Haraba, Viorel Şuică, Felicia Antohe- Effect of folic acid deficient diet and fluvastatin sodium on the expression of caveolin 1 in normal and hyperlipidemic hamsters, Workshop Exploratoriu “De la descoperirile cercetării fundamentale la aplicaţii terapeutice”, Bucureşti, 17 – 18 septembrie 2008.
13. Raluca Haraba, Luminiţa Ivan, Viorel Suica, Elena Uyy, Felicia Antohe - Protein expression of high mobility group box 1 in hyperlipidemic hamsters, Workshop Exploratoriu “De la descoperirile cercetării fundamentale la aplicaţii terapeutice” Bucureşti, 17 – 18 septembrie 2008.
14. Viorel Suică, Luminţa Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe - Proteomica diferenţială a rafturilor lipidice în condiţii normale şi hiperlipidemice, Workshop Exploratoriu “De la descoperirile cercetării fundamentale la aplicaţii terapeutice” Bucureşti, 17 – 18 septembrie 2008.
15. Luminita Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe - Expression of heat shock proteins in lipid loaded endothelial-derived foam cells, 4th International CONFERENCE “The Hsp90 Chaperone Machine” organizata de J. Buchner si D. Picard in Seeon, Germany, 2-6 octomber 2008.
16. Raluca Haraba, Luminita Ivan, Viorel Suica, Elena Uyy, Felicia Antohe - Protein and gene expression of high mobility group box 1 in hyperlipidemic models - 7th International Symposium “Multiple risk factors in cardiovascular diseases prevention and intervention- Health Policy” Venice (Lido), Italy, 22-25 octomber 2008.
17. Elena Uyy, Luminita Ivan, Raluca Haraba, Felicia Antohe – Expression of caveolin 1 in hyperlipidemia: endothelial derived foam cells and hyperlipidemic hamsters, 7th International Symposium “Multiple risk factors in cardiovascular diseases prevention and intervention - Health Policy” Venice (Lido), Italy, 22-25 octomber, 2008.
18. Raluca Haraba, Luminita Ivan, Viorel Suica, Elena Uyy, Felicia Antohe, Maya Simionescu - Expression of high mobility group box 1 protein in Hyperlipidemic models, ICBP 30 years Anniversary Simposium, Bucharest, Romania, 10-12 septembrie 2009.
19. Luminita Ivan, Elena Uyy, Raluca Haraba, Viorel Suica, Felicia Antohe - Expression of heat shok proteins in lipid loaded endothelial-derived foam cells, ICBP 30 years Anniversary Simposium, Bucharest, Romania, 10-12 septembrie 2009.
20. Elena Uyy, Luminita Ivan, Raluca Haraba, Radu Dorel, Felicia Antohe, Maya Simionescu - Upregulation of caveolin-1 expression in pulmonary caveolin-rich membrane domains in diabetes, ICBP 30 years Anniversary Simposium Bucharest, Romania, 10-12 septembrie 2009.
21. Raluca Haraba, Luminita Ivan, Elena Uyy, Viorel Suica, Felicia Antohe, The efect of fluvastatin treatment on HMGB1 in hyperlipidemic animal model, 8th International Congress on Coronary Artery Disease, Prague, Czech Republic, Octombrie 11-14, 2009.
22. Luminita Ivan, Elena Uyy, Raluca Haraba, Viorel Suica, Felicia Antohe, Maya Simionescu, Hyperlipidemia induces endothelial-derived foam cells formation, 8th International Congress on Coronary Artery Disease,Prague, Czech Republic, Octombrie 11-14, 2009.
23. Viorel Suică, Luminita Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe, Proteomic approach of plasma membrane microdomains in experimental hyperlipidemia - 4th Central and Eastern European Proteomics Conference, Viena , Austria, 29.08.2010-1.10. 2010.
LUCRARI PREZENTATE LA MANIFESTARI STIINTIFICE NATIONALE (ABSTRACT)
1. Elena Puchianu, Doina Gazdaru – Studiul spectrofotometric al speciilor de clorofila a in dioxan apos. Sesiunea de comunicari stiintifice a Facultatii de Fizica, Bucuresti, 2001 (comunicare in plen)
2. Luminita Radulescu, Elena Puchianu, Ileana Manduteanu, Felicia Antohe - Analiza proteomica a celulelor endoteliale activate cu seruri umane hiperglicemice si hipercolesterolemice, The Bulletin of the National Society of Cellular Biology, abstract volume 31, 2003.
3. Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe - Prepararea unei fractii membranare imbogatita in caveolina, The Bulletin of the National Society of Cellular Biology, abstract volume 31, 2003.
4. Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe - Expresia caveolinei-1 in fractii membranare pulmonare in conditii normale si patologice, The Bulletin of National Society of Cellular Biology, abstract volume 32, 2004.
5. Felicia Antohe, Luminita Radulescu, Elena Puchianu, Philip Low, Maya Simionescu- Increased uptake of folate conjugates by activated macrophages in experimental hyperlipemia, The Bulletin of National Society of Cellular Biology, abstract, volume 32, 2004.
6. Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe, Maya Simionescu - Imunodetectia caveolinei-1 in fractii membranare pulmonare in soareci cu diabet si hiperlipidemie, A 34-a Conferinta Nationala de Imunologie, Bucuresti, 16-17 septembrie, abstract volume 30, 2004.
7. Felicia Antohe, Luminita Radulescu, Elena Puchianu, Philip S Low, Maya Simionescu - Macrofagele activate in conditii de hipercolesterolemie exprima receptori pentru acid folic. A XXIII-a Sesiune Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Sibiu, 9-12 iunie, Buletin SNBC, nr. 33, p.78, 2005.
8. Elena Puchianu, Luminita Radulescu, Felicia Antohe - Imunodetectia caveolinei 1 in fractii membranare pulmonare de la hamsteri cu ateroscleroza si deficit de acid folic. A XXIII-a Sesiune Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Sibiu, 9-12 iunie, Buletin SNBC, nr. 33, p.147, 2005.
9. Luminita Ivan, Elena Uyy, Felicia Antohe - Expresia proteinelor de soc termic (HSP) in celulele endoteliale incarcate cu lipide. Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Cluj, 9-12 iunie, Buletin SNBC, nr. 36, pag 45, 2008.
10. Elena Uyy, Luminita Ivan, Raluca Haraba, Felicia Antohe – Imunodetectia caveolinei-1 in celulele endoteliale incarate cu lipide si in hamster hiperlipidemic, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Cluj, 9-12 iunie, Buletin SNBC, nr. 36, 2008.
11. Viorel Suica, Luminita Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe –Proteomica diferentiala a rafturilor lipidice in conditii normale si hiperlipidemice, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Cluj, 9-12 iunie, Buletin SNBC, nr. 36, 2008.
12. Raluca Haraba, Luminiţa Ivan, Viorel Suica, Elena Uyy, Felicia Antohe - Expresia proteinei nucleare HMGB1 in hamsteri normali si hiperlipidemici, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Cluj, 9-12 iunie, Buletin SNBC, nr. 36, pag. 47, 2008.
13. Viorel Suica, Luminita Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe - Proteomica diferentiala a rafturilor lipidice in conditii normale si hiperlipidemice, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Bistrita, 9-12 iunie, Buletin SNBC, nr. 37, pag 72, 2009.
14. Luminita Ivan, Elena Uyy, Raluca Haraba, Viorel Suica, Felicia Antohe- Expresia proteinelor de soc termic (HSP) in conditii de hiperlipidemie, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Constanta, 9-12 iunie, Buletin SNBC, nr. 38, pag 234, 2010.
15. Elena Uyy, Felicia Antohe, Luminita Ivan, Raluca Haraba, Dorel L. Radu, Maya Simionescu – Expresia caveolinei-1 in fractii membranarepulmonare din soareci transgenici cu diabet zaharat de tip I, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Constanta, 9-12 iunie, Buletin SNBC, nr. 38, pag 116, 2010.
16. Raluca Haraba, Viorel Suică, Elena Uyy, Luminita Ivan, Felicia Antohe - Caile de semnalizare induse de proteina nucleara HMGB1 in conditii hiperlipidemice, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Constanta, 9-12 iunie, Buletin SNBC, nr. 38, pag 142, 2010.
PrEMII:
1. Premiul “Ion Moraru” de Imunologie Fundamentala – Elena Puchianu, Luminita Radulescu, Doina Popov, Felicia Antohe, “Imunodetectia caveolinei-1 in fractii membranare pulmonare in soareci cu diabet si hiperlipidemie”, A 34-a Conferinta Nationala de Imunologie, – 16-17 Septembrie, Bucuresti, Romania, abstract volume 34, 30-31: 2004 ( comunicare in plen).
2. Premiul III cu posterul: Viorel Suica, Luminita Ivan, Elena Uyy, Raluca Haraba, Felicia Antohe – Proteomica diferentiala a rafturilor lipidice in conditii normale si hiperlipidemice, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SNBC, Bistrita, 9-12 iunie, Buletin SNBC, nr. 37, pag 72, 2009.
3. Premiul III cu posterul: Raluca Haraba, Viorel Suică, Elena Uyy, Luminita Ivan, Felicia Antohe - Caile de semnalizare induse de proteina nucleara HMGB1 in conditii hiperlipidemice, Sesiunea Anuala a Societatii Nationale de Biologie Celulara, Sesiunea Anuala a SRBC, Constanta, 9-12 iunie, Buletin SNBC, nr. 38, pag 142, 2010
ALTE Prezentari STIINTIFICE
1. Viorel Suica, Elena Uyy, Raluca Haraba, Luminita Radulescu, Felicia Antohe, Lipid raft proteomics in hyperlipidemic conditions, Symposium of the COST Action BM0602 WG3 and WG4 Meeting, 13-14 February 2009.
2. Viorel Suica, Elena Uyy, Raluca Haraba, Luminita Radulescu, Felicia Antohe, High resolution proteomic analysis of plasma membrane microdomains, Proteomics Roadshow – BioRad Laboratories, 24 aprilie 2009.
3. Viorel Suica, Elena Uyy, Raluca Haraba, Luminita Radulescu, Felicia Antohe, High resolution proteomic analysis of hyperlipidemic plasma membrane microdomain”, ICBP „N. Simionescu“30 years Anniversary Simposium, Bucharest, Romania, 10-12 septembrie 2009.
4. Luminita Ivan, Elena Uyy, Raluca Haraba, Viorel Suica, Felicia Antohe Proteomic analysis of lipid loaded endothelial-derived foam cells, Al 6-lea Simpozion National de patologie “Patologie celulara si moleculara”, Sesiune Anuala a Institutului National “Victor Babes”, 3-5 noiembrie 2009.
LISTA CONTRACTELOR EXTRABUGETARE Colaborator la urmatoarele proiecte:
2003 - 2005 VIASAN-Modularea secretiei de chemokine in diferite conditii de patologie vasculara; rolul functional al acestora in modelele studiate.
2004 - 2005 CNCSIS - Analiza biochimica si imunologica a fluidelor oculare ca metoda de diagnostic precoce al afectarii analizorului vizual in boli metabolice si reumatismale, director de proiect : dr. Viorica Madalina Cojocaru, Elena Puchianu, 95 milioane lei.
2004 - 2006 VIASAN-Analiza proteomica comparativa a celulelor endoteliale normale si “activate” prin expunere la ser uman hiperlipidemic si hiperglicemic
2005 - 2007 - GAR - Expresia caveolinei 1 in fractii membranare pulmonare in conditii normale si patologice, director de proiect : dr. Felicia Antohe, Elena Puchianu, 85 milioane lei
2004 – 2006 - VIASAN - Analiza proteomica comparativa a celulelor endoteliale normale si “activate” prin expunere la ser uman hiperlipidemic si hiperglicemic, director de proiect: acad. Maya Simionescu, Felicia Antohe,Elena Puchianu, 1,2 miliarde lei.
2005 - 2007 - Grant CEEX colaborare: Cercetari avansate in toleranta imuna cu scopul identificarii de cai terapeutice novatoare in patologia bolilor autoimune de tesut vasculo-conjunctiv. 1 miliard.
2005 - 2008 - Grant CEEX AMCADO IBPC N.S.: Biotehnologii avansate de preparare a unor produse biologice terapeutice de uz uman şi de diagnostic cu integrarea unor metode optimizate de asigurare a calitătii şi de control, în conformitate cu standardele şi normele europene. 500 milioane.
2007- 2010 - PN-II-ID-PCE-2007 colaborare: Mecanisme celulare si moleculare implicate in dezvoltarea placii aterosclerotice.
Data:
25.10. 2010
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