icbp

Coordinator: Victor Jinga, M.D., Ph.D.

Endothelial-derived foam cells obtained in culture (V. V. Jinga)

Role

■ Establishment and characterization of original pure cell lines; cryopreservation
■ Growing and cryopreservation of imported cell lines
■ Development of new procedures for cell isolation, selection and culture for in vitro studies
■ Technical methods for cell cultures

The ICBP’s cell culture collection contains over 75 different cell lines, (original and/or expanded in the laboratory). Recently, a bank of human osteoprogenitor cell units, derived from bone morrow was started up for the use in bone pathology and traumatology as autotransplant. The cell culture collection is currently utilized in numerous projects.

Some original pure diploid cells lines available for research and produced in ICBP "Nicolae Simionescu"

■ E-AB-10 Aortic endothelial cells derived from bovine aorta by collagenase digestion (original procedure)

■ E-AB-13 and E-AB-93. Bovine aortic endothelial cells, derived by trypsin digestion (original procedure)

■ PE-CS Bovine pulmonary artery endothelial cells, derived by trypsin digestion (original procedure)

■ M-AB-7, M-AB-8, M-AB-83, M-AB-93-I (II). Bovine aortic smooth muscle cells derived by explantation

■ PM-CS. Bovine pulmonary smooth muscle cell (explantation)

■ M-CA 2+3, M-CA 4. Simian (Cercopithecus Africanus) aortic smoot muscle cells (explantation)

■ H-Ao SM/I IM, H-AoSM/II IM. Human fetal aortic smooth muscle cells (explantation)

■ MAI-IM. Rabbit aorta smooth muscle cells (explantation)

■ M2SM-TM. Rat aorta smooth muscle cells (explantation)

■ HPEC. Human placental endothelial cells (enzymatic perfusion, differential centrifugation; original procedure)

■ F-HD1. Dermal human fibroblast (explantation from a patient with cirrhosis)

■ F-HD2. Dermal human fibroblast (LDL-receptor defective) (explantation)

■ CEV-ILM. Bovine cardiac valvular endothelial cells (non-enzymatic-EDTA-detachment)  (original procedure)

■ Rat vascular pericytes (enzymatic dispersion)

■ HAEC. Hamster aortic endothelial cells (collagenase digestion)

■ BMSC. Bone marrow stromal cells

■ MAEF. Mouse embryonic fibroblasts

■ mESC. Mouse embryonic stem cells

■ CMC. Rat cardiomyocites. Primary culture

■ MSC. Mouse mesenchymal stem cells (bone marrow)