• Lipoproteins characterization: alterations in atherosclerosis and diabetes; transport through vascular endothelium; implication in atheroma formation; interaction with cells of the arterial wall.
• Lipoproteins Receptors: gene and protein expression in various organs and cell culture
Animal models
• Cellular biology and biochemistry of blood vessels, heart and cardiac valves in normal and pathological conditions.
• Experimental models of atherosclerosis and/or diabetes
• Modification of the arterial wall and heart valves in the prelesional and lesional stage of experimentally induced atherosclerosis
• Pathobiochemistry of diabetes and diabetes induced - accelerated vascular diseases
In vitro models
Cell cultures: human Endothelial Cells, Smooth Muscle Cells, monocyte/macrophages THP-1, hepatoma HepG2. |
Analysis in human subjects
- lipid and lipoprotein parameters in plasma
- lipid transfer enzymes
- antioxidant potential
- light and electron microscopy of various tissues
- immunohystochemistry
Technical Expertise
Lipoprotein isolation: ultracentrifugation.
Biochemical assays: polyacrilamide and agarose electrophoresis, immunoelectrophoresis, isoelectric focusing, gel filtration-, affinity- and ion exchange chromatography, thin layer chromatography, fluorescence microscopy, enzymes kinetics.
Microscopy: electron, fluorescence and light microscopy, immunohistochemistry of vessel wall.
Molecular biology: genotyping, gene expression, protein expression - identification and analysis of proteins, transfection, PCR and Real-Time PCR, silencing RNA
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