About Us

Institute of Cellular Biology and Pathology

Gene Regulation and Molecular Therapies

Gene regulation and molecular therapies research team

We are currently working in the field of atherosclerosis, focusing on therapeutic strategies and the detailed study of lipid metabolism. We use molecular tools and in vitro and in vivo models to explore the roles of various apolipoproteins in modulating lipoprotein function, inflammatory signaling, and metabolic cross-talk, including their impact on glucose metabolism and mitochondrial function in energy production.

Through our research, we aim to connect fundamental discoveries with real-world therapeutic advances in cardiovascular medicine. By integrating molecular insights with translational strategies, we attempt to shape innovative approaches to combat vascular diseases.

Facilities

Research Infrastructure

The research infrastructure is updated and includes the equipment needed for molecular biology (cloning, PCR, Real Time PCR, ChIP, electroporation, transfections), biochemistry (western blot, ELISA, spectrophotometric and luminescent assays), cell culture, adeno- and lentiviral production, packaging and transduction, cell encapsulation, and lipoprotein fraction analysis (FPLC and Lipoprint).

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Networks

Collaborative Research

Our group has been deeply involved in collaborative research, spanning academic and industry partners across Europe and the United States.

CARDIOSCOPE — HORIZON-MSCA-2021-SE-01-01

Grant Agreement n° 101086397 · 2023–2026 · led by Dr. Paolo Magni, University of Milan

Comprehensive and personalized assessment of acute coronary syndrome by multiomic approach and artificial intelligence strategy. An impressive opening between academic and private companies, and an excellent opportunity for collaborative research.

STROMA — PNRR project (contract 258/2023)

Led by Dr. Kyriakos Kypreos

Strengthening Romanian competence in metabolic syndrome research by exploring the role of apolipoprotein A2 in dyslipidemia, obesity, and type II diabetes mellitus. A fruitful collaboration between our team and Dr. Kypreos’ team.

ERA-NET PerMed — 2019–2022

A. Gafencu, Coordinator for the Romanian team

Integrative Personal Omics Profiles in Glioblastoma Recurrence and Therapy Resistance.

University of Crete — since 2002

Prof. Dimitris Kardassis

Ongoing collaboration begun when Anca Gafencu was a visiting fellow in Prof. Kardassis’ lab under a NATO Science grant — Collaborative linkage grant: “Role of ApoE in cholesterol and triglyceride homeostasis”, awarded to Prof. Vassilis Zannis, Boston University School of Medicine, USA.

COST Actions

Previously part of HDLNet (BM0904). Actively participating in AtheroNet (CA21153) and CardioPharmaGENET (CA24165).

Events

Scientific Meetings Organized

2007 Advances in Gene Regulation — contract 016873/2005, SERA
2008 From Gene Expression and Protein Activation to Human Diseases — contract 016873/2005, SERA
2012 Simionescu Advanced School of Cellular and Molecular Approaches for the Progress of the Biomedical Research — Nov 5–14, supported by UEFISCDI
2013 “HDL Physiology, Regulation and Therapeutic Potential” — Bucharest & Sinaia, Aug 26–30, COST Action BM0904
2021 Encapsulation of Cells and Drugs: Materials, Processes, and Applications — project INTERA1
2023 First COST Action CA21153 AtheroNet MC & WG Meeting — Bucharest, March 21–24 (Network for implementing multiomics approaches in atherosclerotic cardiovascular disease prevention and research)

Funding History · 1996–2026

Research Grants & Projects

2022–24

“A novel anti-atherosclerotic chimeric protein that impedes the infiltration of the inflammatory cells in the vessel wall”

PN-III-P4-PCE-2021-1755 · Project Director A. Gafencu

2020–22

Demonstration of the enhanced anti-atherosclerotic potential of apolipoprotein E targeted to the activated endothelium, by fusion with VCAM-1 binding peptide

PN-III-P2-2.1-PED-2019-4574 · Project Director A. Gafencu

2019–22

Integrative Personal Omics Profiles in Glioblastoma Recurrence and Therapy Resistance

ERA-NET PerMed · A. Gafencu, Coordinator for the Romanian team

2016–20

Improve institutional competitiveness in the field of type 1 diabetes by developing an innovative concept of immunotherapy based on mesenchymal stromal cells (DIABETER)

Project Director Nadir Askenasy

2015–17

Genetically engineered apolipoproteins immobilized on nanoparticles: a Molecular Trojan horse targeting atherosclerotic plaque (APGEN)

PN-II-RU-TE-2014-4-2143, CNCSIS Romania · Project Director A. Gafencu

2011–16

Apolipoprotein E-based novel anti-atherosclerosis cell-therapy approaches

PN-II ID_PCE-2011-3-0591, CNCSIS Romania · Project Director A. Gafencu

2009–11

Novel strategies employing genetic engineering to increase plasma HDL, the protective lipoproteins in atherosclerosis

Exploratory research project 1307, CNCSIS Romania · Project Director A. Gafencu

2005–07

Regulation of apolipoprotein gene clusters — an innovative therapeutic approach of neurodegenerative and cardiovascular diseases

Romanian Ministry of Research, Excellence Research Program: Projects for Young Researchers · Project Director A. Gafencu

2005–07

Regulation of the expression of the human apolipoprotein E gene in macrophages and the brain: new approaches for the treatment of dyslipidemias and Alzheimer’s disease

Greek & Romanian Ministries of Research · Prof. Dimitris Kardassis & Dr. Anca Gafencu

2003–05

ApoE gene regulation — therapeutic target in cardiovascular and neurodegenerative diseases

National Research Program for Biotechnology “BIOTECH” · Project Director A. Gafencu

2001–03

Characterization of albumin and IgG receptors, expressed by the endothelial cells in normal and pathologic states

National Research Program “CERES” · Project Director A. Gafencu

2000

Functional analysis of the IgG receptors in endothelial cells

Romanian Research Ministry · Project Director A. Gafencu

1999

Identification of FcR in human placental endothelial cells

Romanian Academy · Project Director A. Gafencu

1998

Immortalization of endothelial cells

Romanian Academy · Project Director A. Gafencu

1997–98

Lipid composition of the microdomains of the endothelial cell membrane

Romanian Academy · Project Director A. Gafencu

1996–99

Biochemical and functional characterization of the endothelial plasmalemma

Romanian Research Ministry · Project Director A. Gafencu

Gene regulation and molecular therapies