Research  »  DEP
PROTEOMICS

 

 

RESEARCH STRATEGY

Integration of the biomedical research into the modern precision medicine.

Using smart approaches based on mass spectrometry proteomics and wet laboratory methods we aim to develop translational personalized biomedicine. Uncover the inter-relation of basic structural and functional proteomics with clinical patients’ profile will lead to early diagnostic, prevention and adequate personalized therapies.

 

 

Major research interests of the department:

  • The application of mass spectrometry-based proteomics for biomarker research in chronic non-communicable diseases (atherosclerosis, type 1 and 2 diabetes, gestational diabetes, diabetic foot syndrome, cancer);
  • The investigation of DAMPs as potential predictors and therapeutic targets in inflammatory pathologies;
  • Identification of markers and mechanisms involved in cardiac recovery post-myocardial infarction;
  • Uncovering mechanisms of early atherosclerosis and/or plaque instability in coronary artery disease;
  • Membrane microdomains (lipid rafts, plasmalemmal vesicles, caveolae): structural and functional characterization;
  • Transport of macromolecules in vascular cells: cell receptors, transcytosis, endocytosis.

 

 

Staff:

Felicia Antohe, Ph.D., Head, Scopus ID: 6602183914

Luminita (Radulescu) Ivan, Ph.D., Scopus ID: 35752182600 and 55675223141

Elena (Puchianu) Uyy, Ph.D., Scopus ID: 36097083900 and 8220623100

Raluca Maria (Haraba) Boteanu, Ph.D., Scopus ID: 55967352000

Viorel Iulian Suica, Ph.D., Scopus ID: 37121178700

Mirel Adrian Popa, PhD., Scopus ID: 55790189100

Diana Valentina Uta, PhD Student

 

Felicia Antohe, Ph.D., CS I

Major position/appointments and professional training:

Member of the Scientific Council of ICBP “Nicolae Simionescu”

Ph.D. Coordinator; Advisor for Graduate and Master Programs

Research fellow: • McGill University, Montreal, Canada.

Visiting scientist: • University of Alberta, Edmonton Canada; Max Planck Institute, Bad Nauheim, Germany; University of Texas, Dallas, USA; • Molecular Biology and Genetics, Dresden, Germany; Université René Descartes, Paris V, France; L’Université des Sciences et Technologies de Lille I, France.

Expert Evaluator/Reporter: • European Community Framework; Ministry of Education and Research (MER); National Council of Research and High Education: National Framework for Research, Development and Innovation; Invited peer reviewer for international journals.

President of the Commission of MER for high academic degrees, CNATDCU (2006-2011).

Training, mentoring and international position: 

  • Romanian Representative of the Central and Eastern European Proteomic Conference (CEEPC). www.ceepc.eu.
  • Member of the Management Comity of the EU FPH COST Action “CliniMARK CA” 16113. www.cost.eu/COST

 

 

 

EERTIS infrastructure and services: https://eertis.eu/erlb-2300-000n-0714

 

 

 

Doctoral studies / Ph.D. SCSAAR program

  • 2023-present  Diana Valentina Uta, Ph.D. supervisor Dr. Felicia Antohe
  • 2017-2024      Elena Georgiana Bernea, Ph.D. supervisor Dr. Felicia Antohe and co-supervisor Acad. Constantin Ionescu-Târgoviște
  • 2010-2015      Brandusa Elena Lixandru, supervisor Dr. Felicia Antohe
  • 2014-2018      Baciu Anca, supervisor Dr. Felicia Antohe and co-supervisor Prof Univ. Aurel Popescu
  • 2008-2013      Andrei Constantinescu, supervisors Dr. Felicia Antohe and Acad. Maya Simionescu
  • 2008-2014      Viorel Iulian Suica, supervisor Dr. Felicia Antohe
  • 2007-2016      Daniela Cristea, supervisor Dr. Felicia Antohe
  • 2007-2014      Mihaela Oprea, supervisor Dr. Felicia Antohe
  • 2006-2012      Carmen Iordache, supervisor Dr. Felicia Antohe
  • 2005-2012      Raluca Maria Boteanu, supervisor Dr. Felicia Antohe
  • 2003-2012      Elena Uyy, supervisor Dr. Felicia Antohe
  • 2002-2007      Madalina Viorica Cojocaru supervisor Dr. Felicia Antohe

 

 

PRIDE (ISI Proteomics Identification Database):

  1.  PXD033683 - Short-term blockade of pro-inflammatory alarmin S100A9 favorably modulates left ventricle proteome and related signaling pathways involved in post – myocardial infarction recovery. 2022-05-19; PRIDE (Proteomics Identification Database) 2022
  2. PXD035692 - Coronary alarmins as residual risk markers of atherosclerosis under hypolipidemic therapy. 2022-10-03; PRIDE (Proteomics Identification Database) 2022
  3. PXD035673 - Exosome proteomics reveals the deregulation of coagulation, complement and lipid metabolism proteins in gestational diabetes mellitus. 2022-09-22; PRIDE (Proteomics Identification Database) 2022
  4. PXD026379 - Alteration of DAMPs and regulated cell death under hyperlipidemic stress. 2022-02-22; PRIDE (Proteomics Identification Database) 2022
  5. PXD026380 - Proteomic Analysis of Estrogen Mediated Enhancement of MSC-Induced Angiogenesis in vivo. 2022-02-17; PRIDE (Proteomics Identification Database) 2022
  6. PXD016135 - Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model. 2021-04-29; PRIDE (Proteomics Identification Database) 2021

 

 

 

Recent findings

 


Hepatic Alarmins and Mitochondrial Dysfunction under Residual Hyperlipidemic Stress Led to Irreversible NAFLD

 

The work flow strategy to identify the main signaling pathways and liver proteome changes induced by hypercholesterolemia in a rabbit atherosclerotic model that determined high accumulation of lipids in the liver. The effect of combined lipid-lowering drugs (statins and anti-PCSK9 monoclonal antibody) were used after the interruption of the hypercholesterolemic diet to identify also the potential mediators, such as alarmins, responsible for the irreversible NAFLD build up under the hyperlipidemic sustained stress. Mitochondrial dysfunction indicated alteration at the mitochondrial respiratory chain level and down-regulation of NADH: ubiquinone oxidoreductase. The expression of cytochromes (P4502E1, b5, and c) were up-regulated by lipid-lowering treatment. Long-term hyperlipidemic stress, even with a low-fat diet and lipid-lowering treatment, was accompanied by alarmin release (annexins, galectins, HSPs, HMGB1, S100 proteins, calreticulin, and fibronectin) that generated local inflammation and induced liver steatosis and aggressive fibrosis (by high abundance of galectin 3, fibronectin, and calreticulin).

DOI: 10.14218/JCTH.2022.00128

 

 

 

 

Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus

 

 

Control subjects (CG) and gestational diabetes mellitus patients (GDM) were used to characterize the serum exosomal protein cargo and associated signaling pathways. Label-free relative quantification was followed by bioinformatic pathway analysis. Pearson correlation was pursued to associate the regulation trend of the differentially abundant molecules with clinical and paraclinical variables. The proteomics analysis revealed 78 significantly altered proteins when comparing GDM to CG, related to complement and coagulation cascades, platelet activation, prothrombotic factors and cholesterol metabolism. Down-regulation of Complement C3 (C3), Complement C5 (C5), C4-B (C4B), C4b-binding protein beta chain (C4BPB) and C4b-binding protein alpha chain (C4BPA), and up-regulation of C7, C9 and F12 were found in GDM. Our data indicated significant correlations between factors involved in the pathogenesis of GDM and clinical parameters that may improve the understanding of GDM pathophysiology.

DOI: 10.3390/molecules27175502

 

 

 

Short-Term Blockade of Pro-Inflammatory Alarmin S100A9 Favorably Modulates Left Ventricle Proteome and Related Signaling Pathways Involved in Post-Myocardial Infarction Recovery

 

We hypothesized that a S100A9 blockade induces changes of major signaling pathways implicated in post-MI healing. Mice were subjected to myocardial infarction (MI) by permanent left coronary artery ligation. The S100A9 blocker (ABR-23890) was given for 3 days after the procedure. At 3- and 7-days post-MI, ventricle samples were analyzed versus control and Sham-operated mice. Blockade of S100A9 modulated the expressed proteins involved in five biological processes: leukocyte cell-cell adhesion, regulation of the muscle cell apoptotic process, regulation of the intrinsic apoptotic signaling pathway, sarcomere organization and cardiac muscle hypertrophy. These processes could be valuable new pharmacological targets for the treatment of ischemic heart.

DOI: 10.3390/ijms23095289

 

 

 

Regulated cell death joins in atherosclerotic plaque silent progression

We hypothesized that DAMPs and non-apoptotic regulated cell death are critical players of atherosclerotic plaque progression with inadequate response to lipid-lowering treatment. We aimed to uncover the silent mechanisms that govern the existing residual risk of cardiovascular-related mortality in experimental atherosclerosis. Proteomic and genomic approaches were applied on the ascending aorta of hyperlipidemic rabbits and controls with and without lipid-lowering treatment. The hyperlipidemic animals, which presented numerous heterogeneous atherosclerotic lesions, exhibited high concentrations of serum lipids and increased lipid peroxidation oxidative stress markers. The analyses revealed the significant upregulation of DAMPs and proteins implicated in ferroptosis and necroptosis under hyperlipidemia.

DOI: 10.1038/s41598-022-06762-y

 

 

 

Cardiac Alarmins as Residual Risk Markers of Atherosclerosis under Hypolipidemic Therapy

 

Although statin treatment can effectively lower the main atherosclerotic risk factor (increased levels of low-density lipoproteins), there is still a residual threat of cardiovascular events. We hypothesized that a specific panel of stress-sensing molecules (alarmins) could indicate the persistence of silent atherosclerosis residual risk. Mass spectrometry experiments of left ventricle lysates harvested from control, atherosclerotic and treated atherosclerotic New Zealand White rabbits were complemented by immunologic and genomic studies to corroborate the data. The hyperlipidemic diet determined a general alarmin up-regulation tendency over the control group. A significant spectral abundance increase was measured for specific heat shock proteins, S100 family members, HMGB1, and Annexin A1. The hypolipidemic treatment demonstrated a reversed regulation trend with non-significant spectral alteration over the C group for some of the identified alarmins.

DOI: 10.3390/ijms231911174

 

 

 

 

Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model

 

Due to their excellent mechanical and biocompatibility properties, titanium-based implants are successfully used as biomedical devices. However, when new bone formation fails for different reasons, impaired fracture healing becomes a clinical problem and affects the patient's quality of life. We aimed to design a new bioactive surface of titanium implants with a synergetic PEG biopolymer-based composition for gradual delivery of growth factors (FGF2, VEGF, and BMP4) during bone healing. The optimal architecture of non-cytotoxic polymeric coatings deposited by dip coating under controlled parameters was assessed both in cultured cells and in a rat tibial defect model (100% viability). Notably, the titanium adsorbed polymer matrix induced an improved healing process when compared with the individual action of each biomolecule. High-performance mass spectrometry analysis demonstrated that recovery after a traumatic event is governed by specific differentially regulated proteins, acting in a coordinated response to the external stimulus. The proposed functional polymer coatings of the titanium implants demonstrated the significant improvement of bone healing process after injury.

DOI: 10.1038/s41598-020-75527-2

 

 

 

Proteomic Analysis of Estrogen-Mediated Enhancement of Mesenchymal Stem Cell-Induced Angiogenesis In Vivo

Therapeutic use of mesenchymal stem cells (MSCs) for tissue repair has great potential. MSCs from multiple sources, including those derived from human umbilical matrix, namely Wharton’s jelly, may serve as a resource for obtaining MSCs. However, low in vivo engraftment efficacy of MSCs remains a challenging limitation. To improve clinical outcomes using MSCs, an in-depth understanding of the mechanisms and factors involved in successful engraftment is required. Using a proteomics approach, we investigated the angiogenic potential of MSCs in vivo and the modulatory actions of 17β-estradiol on mechanisms involved in tissue repair. The LC-MS screening of proteins obtained from the excised Matrigel plugs from ovariectomized mice revealed 71 proteins that were significantly altered following E2 exposure, 57 up-regulated proteins and 14 down-regulated proteins. A major result was the association of over 100 microRNA molecules (miRNAs) involved in cellular communication, vesicle transport, and metabolic and energy processes, and the high percentage of approximately 25% of genes involved in unknown biological processes. Together, these data provide evidence for increased angiogenesis by MSCs treated with the sex hormone 17β-estradiol. In conclusion, 17β-estradiol treatment may increase the engraftment and repair potential of MSCs into tissue, and may promote MSC-induced angiogenesis after tissue injury.

DOI: 10.3390/cells10092181

 

 

 

Endoplasmic Reticulum Chaperones Are Potential Active Factors in Thyroid Tumorigenesis

 

 

The study aimed to evaluate the proteomic changes in benign follicular adenoma versus malignant follicular variant of papillary thyroid carcinoma. Tumor and nontumor adjacent samples were analyzed by liquid nano-chromatography mass spectrometry, and protein abundance was evaluated by label-free quantification. Western blotting and quantitative real-time polymerase chain reaction were used to validate and complement the mass spectrometry data. The results demonstrated deregulated expression of four endoplasmic reticulum chaperones (78 kDa glucose-regulated protein, endoplasmin, calnexin, protein disulfide-isomerase A4), glutathione peroxidase 3 and thyroglobulin, all of them involved in thyroid hormone synthesis pathway. The altered tissue abundance of endoplasmic reticulum chaperones in thyroid cancer was correlated with serum expression levels. The identified proteins significantly discriminate between adenoma and carcinoma in both thyroid tissue and corresponding sera

DOI: 10.1021/acs.jproteome.6b00567

 

 

 

Publications (2014-2025)

 

2025

Cardiac ATP production and contractility are favorably regulated by short-term S100A9 blockade after myocardial infarction, Boteanu RM, Suica VI, Uyy E, Ivan L, Mares RG, Simionescu M, Schiopu A, Antohe F, Journal of Advanced Research, 2025, IF: 11.4

 

2024

Short‑term S100A8/A9 Blockade Promotes Cardiac Neovascularization after Myocardial Infarction, J Cardiovasc Transl Res. RG Mares*, VI Suica*, E Uyy, RM Boteanu, L Ivan, IG Cocuz,A H Sabau,Vikas Yadav, I A Szabo, O Simion Cotoi, M E Tomut, G Jakobsson, M Simionescu, F Antohe*, A Schiopu*, (*equal contribution) 2024 Jul 15. Journal of Cardiovascular Translational Research, 2024, IF:2.4

Dihydrotestosterone Augments the Angiogenic and Migratory Potential of Human Endothelial Progenitor Cells by an Androgen Receptor-Dependent Mechanism Popa MA, Mihai CM, Șuică VI, Antohe F, Dubey RK, Leeners B, Simionescu M. 2024 Apr 29;25(9):4862. Int J Mol Sci. 2024, IF: 4.9

 

2023

Hepatic Alarmins and Mitochondrial Dysfunction under Residual Hyperlipidemic Stress Lead to Irreversible NAFLD, L Ivan, E Uyy, VI Suica, RM Boteanu, A Cerveanu-Hogas, R Hansen, F Antohe, Volume 11, Issue 2, Pages 284-294, Published 2023/4/28, Journal of Clinical and Translational Hepatology, 2023 IF: 5.065

Biotechnological advances and applications of human pluripotent stem cell-derived heart models Muniyandi P, O'Hern C, Popa MA, Aguirre A. Published 2023 Jul 25;11:1214431. Front Bioeng Biotechnol, 2023, IF: 4.3

 

2022

Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus, EG Bernea*, VI Suica*, E Uyy*, A Cerveanu-Hogas, RM Boteanu, L Ivan, I Ceausu, DA Mihai, C Ionescu-Tîrgoviște, F Antohe, (*equal contribution) Volume 27, Issue 17, Pages 5502, Published 2022/8/26 Molecules, 2022 IF:4.6

Short-Term Blockade of Pro-Inflammatory Alarmin S100A9 Favorably Modulates Left Ventricle Proteome and Related Signaling Pathways Involved in Post-Myocardial Infarction Recovery, RM Boteanu, VI Suica, E Uyy, L Ivan, A Cerveanu-Hogas, RG Mares, M Simionescu, A Schiopu, F Antohe, Volume 23, Issue 9, Pages 5289, Published 2022/5/9 International Journal of Molecular Sciences 2022 IF:5.6

New born macrosomia in gestational diabetes mellitus, EG Bernea*, E Uyy*, DA Mihai, I Ceausu, C Ionescu‑Tirgoviste, VI Suica, L Ivan, F Antohe, (*equal contribution) Volume 24, Issue 6, Pages 1-12, Publication date 2022/12/1 Experimental and Therapeutic Medicine 2022 IF:2.7

Regulated cell death joins in atherosclerotic plaque silent progression, E Uyy, VI Suica, RM Boteanu, A Cerveanu-Hogas, L Ivan, R Hansen, F Antohe; Volume 12, Issue 1, Pages 1-14, Published2022/2/18 Journal Scientific Reports, 2022 IF:4.6

Cardiac Alarmins as Residual Risk Markers of Atherosclerosis under Hypolipidemic Therapy, VI Suica, E Uyy, L Ivan, RM Boteanu, A Cerveanu-Hogas, R Hansen, F Antohe, Volume 23, Issue 19, Pages 11174, Publication date 2022/9/22 International Journal of Molecular Sciences 2022 IF:5.6

Fibroblast growth factor receptor-2 modulates the MMP2 and MMP14/TIMP1 ratio in human cardiac myofibroblasts, Popa, M. A., C. M. Mihai, F. Antohe, and M. Simionescu. Febs Open Bio, vol. 12, pp. 43-43. 111 River St, Hoboken 07030-5774, Nj Usa: Wiley, 2022. IF: 2.6

Changes in Serum Insulin and Proinsulin Levels in Gestational Diabetes-A Brief Review, E G Bernea, I Ceausu, DE Georgescu, C Ionescu-Tirgoviste, F Antohe, DA Mihai 23, 113 Research&Science Today 2022

Serum Levels of Adiponectine and Leptin in Gestational Diabetes Mellitus-Review EG Bernea, I Ceausu, Dragos E Georgescu, C Ionescu-Tirgoviste, F Antohe, DA Mihai Journal Nr 1, Volume 23, Pages 131 Research&Science Today 2022

 

 2021

Proteomic Analysis of Estrogen-Mediated Enhancement of Mesenchymal Stem Cell-Induced Angiogenesis In Vivo. Mihai MC, Popa MA, Șuică VI, Antohe F, Jackson EK, Leeners B, Simionescu M, Dubey RK. 10(9):2181. Published: Aug 24 2021 Cells. 2021 IF: 7.666

Inflammatory status of the pancreas in NOD mice that do not develop overt diabetes Catrina AM, Popa MA, Văcaru AM, Fenyo IM. Published 2021 Jan-Mar;62(1):109-115. Rom. J Morphol Embryol, 2021, IF: 0.833

 

 2020

Proteomics Of Regenerated Tissue In Response To A Titanium Implant With A Bioactive Surface In A Rat Tibial Defect Model, RM Boteanu, VI Șuică, L Ivan, F Safciuc, E Uyy, E Drăgan, SM. Croitoru, V Grumezescu, M Chirițoiu, LE. Sima, C Vlăgioiu, G Socol, F Antohe, Volume: ‏ 10Issue: ‏ 1Article Number: 18493 Published: ‏oct 28 2020 Scientific Reports 2020 IF: 4.38

Diabetic Nephropathy Associates With Deregulation Of Enzymes Involved In Kidney Sulphur Metabolism, E Uyy, VI Șuică, RM Boteanu, F Safciuc, A Cerveanu-Hogaș, L Ivan, C Stavaru, M Simionescu, F Antohe, Volume: ‏ 24Issue: ‏ 20Pages: ‏ 12131-12140 Published: oct 2020 Journal of Cellular and Molecular Medicine 2020 IF: 5.31

Analytical techniques for multiplex analysis of protein biomarkers, Van Gool A, Corrales F, Čolović M, Krstić D, Oliver-Martos B, Martínez-Cáceres E, Jakasa I, Gajski G, Brun V, Kyriacou K, Burzynska-Pedziwiatr I, Wozniak LA, Nierkens S, Pascual García C, Katrlik J, Bojic-Trbojevic Z, Vacek J, Llorente A, Antohe F, Suica V, Suarez G, t'Kindt R, Martin P, Penque D, Martins IL, Bodoki E, Iacob BC, Aydindogan E, Timur S, Allinson J, Sutton C, Luider T, Wittfooth S, Sammar M, Volume: ‏ 17 Issue: ‏ 4 Pages: ‏ 257-273Published: ‏APR 2 2020 Expert Review of Proteomics 2020 IF: 3.940

Long-Term Evaluation of Dip-Coated PCL-Blend-PEG Coatings in Simulated Conditions, A. I. Vişan, G. Popescu-Pelin, O. Gherasim, A. Mihăilescu, M. Socol, I. Zgura, M. Chiriţoiu, L. E. Sima, F. Antohe, L. Ivan, D. M. Vrânceanu, C. M. Cotruţ, R. Cristescu, and G. Socol, Volume: ‏ 12Issue: ‏ 3Article Number: 717 Published: ‏mar2020 Polymers 2020 IF: 4.329

Management of gestational diabetes-a review of current literature. EG Bernea, F Antohe, A Mihai, I Ceaușu, C Guja, C Ionescu-Tîrgoviște, Issue 1, Publication date 2020/3/1 Research&Science Today 2020

Biogenesis of Exosomes-A Review of Current Literature, EG Bernea, A Mihai, C Guja, C Ionescu-Tirgoviste, F Antohe, A Pietrosel, Volume 20, Pages 39, Publication date 2020" Research&Science Today 2020

Maternal and Fetal Complications of Gestational Diabetes-Review, EG Bernea, A Mihai, A Pietrosel, I Ceausu, F Antohe, C Ionescu-Tirgoviste, Volume 20, Pages 55, Publication date 2020 Research&Science Today 2020

 

 2019

Mechanism of 17 beta-estradiol stimulated integration of human mesenchymal stem cells in heart tissue, M. C. Mihai, M. A. Popa, V. I. Şuică, F. Antohe, E. K. Jackson, M. Simionescu, and R. K. Dubey, Volume: ‏ 133 Pages: ‏ 115-124 Published: ‏ AUG 2019 Journal of Molecular and Cellular Cardiology 2019 IF: 4.133

Driving Precision Medicine through Proteomics and Metabolomics-12th Central and Eastern European Proteomic Conference (CEEPC), Bucharest, Romania, S. J. Gadher, F. Antohe, and H. Kovarova, Volume: ‏ 16 Issue: ‏ 7 Pages: ‏ 549-552 Published: ‏Jul 3 2019 Expert Review of Proteomics 2019 IF: 3.614

Evaluation of the immunogenicity of reverse-vaccinology derived staphylococcus aureus proteins on experimental endocarditis model, M Oprea, B Cordoş, M Străut, A Costache, F Antohe, A Onu, Volume 78, Issue 1, Pages 5-16, Publication date 2019" Romanian Archives of Microbiology and Immunology 2019

In Vivo Pharmacological and Anti-inflammatory Evaluation of Xerophyte Plantago sempervirens Crantz Farcaș AD, Moț AC, Pârvu AE, Toma VA, Popa MA, Mihai MC, Sevastre B, Roman I, Vlase L, Pârvu M. 2019 Jun 2; 2019: 5049643. Oxid Med Cell Longev, 2019, IF: 5.076

 

 2018

Dihydrotestosterone induces pro-angiogenic factors and assists homing of MSC into the cardiac tissue, M. A. Popa, M. C. Mihai, A. Constantin, V. I. Şuică, C. Ţucureanu, R. Costache, F. Antohe, R. K. Dubey, and M. Simionescu, Volume: ‏ 60 Issue: ‏ 1Pages: ‏ 1-15 Published: ‏Jan 2018 Journal of Molecular Endocrinology 2018, IF: 3.744

Oxidative stress and gestational diabetes mellitus. The effects of supplements on oxidative stress, EG Bernea, F Antohe, A Mihai, C Ionecsu-Tirgoviste, Proc Rom Acad Ser B, Volume 20, Issue 2, Pages 121-131, 2018 Proceedings Of The Romanian Academy 2018

 

 2017

Proteomic analysis of plasma molecular markers as predictors of differentiated thyroid cancer, A. E. Baciu*, E. Uyy*, V. I. Şuică*, R. M. Boteanu, A. I. Popescu, C. Giulea, D. Manda, C. Badiu, and F. Antohe, (*equal contribution) Volume: ‏ 69 Issue: ‏ 1 Article Number: 601 Published: ‏ 2017Romanian Reports in Physics 2017 IF: 1.582

High Resolution Mass Spectrometry Analysis Of Ribosomal Proteins In Experimental Diabetes, Uyy, E; Suica, VI; Boteanu, RM; Ivan, L; Safciuc, F; Antohe, F, Volume: ‏ 69 Issue: ‏ 1 Article Number: 602 Published: ‏ 2017Romanian Reports in Physics 2017 IF: 1.582

Alarmins in chronic noncommunicable diseases: Atherosclerosis, diabetes and cancer, R. M. Boteanu*, V. I. Şuică*, E. Uyy, L. Ivan, S. O. Dima, I. Popescu, M. Simionescu, and F. Antohe, (*equal contribution) Volume: ‏ 153 Special Issue: ‏ SI Pages: ‏ 21-29 Published: feb 5 2017 Journal of Proteomics 2017 IF: 3.722

Laser deposition of poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) - lysozyme microspheres-based coatings with anti-microbial properties, V. Grumezescu, A. M. Holban, L. E. Sima, M. B. Chiriţoiu, G. N. Chiriţoiu, A. M. Grumezescu, L. Ivan, F. Safciuc, F. Antohe, C. Florica, C. R. Luculescu, M. C. Chifiriuc, and G. Socol Volume: ‏ 521Issue: ‏ 1-2Pages: ‏ 184-195Published: ‏APR 15 2017 International Journal of Pharmaceutics 2017 IF: 3.862

 

 2016

Endoplasmic Reticulum Chaperones Are Potential Active Factors in Thyroid Tumorigenesis, E. Uyy*, V. I. Şuică*, R. M. Boteanu, D. Manda, A. E. Baciu, C. Badiu, and F. Antohe, (*equal contribution) Volume: ‏ 15Issue: ‏ 9Pages: ‏ 3377-3387 Published: sep 2016 Journal of Proteome Research 2016 IF: 4.268

Comparative proteomic analysis of membrane microdomains isolated from two hyperlipidemic animal models, V. I. Şuică, E. Uyy, R. M. Boteanu, L. Ivan, and F. Antohe, Volume: ‏ 1864Issue: ‏ 9Pages: ‏ 1061-1071 Published: ‏sep 2016 Biochimica et Biophysica Acta-Proteins and Proteomics 2016 IF: 2.773

Preliminary structural ultrasonic characterization of hydroxyapatite, C. L. Popa, S. Antohe, F. Safciuc, MEC. El Kettani, D. Leduc, F. Antohe, and MV. Predoi, Volume: ‏ 11Issue: ‏ 3Pages: ‏ 997-1006 Published: ‏ iul-sep 2016 Digest Journal of Nanomaterials and Biostructures 2016, IF: 0.836

Prevalence of virulence markers and pHS-2-like plasmids among Shigella sonnei and Shigella flexneri isolates originating from shigellosis cases in Romania, D. Cristea, M. Oprea, A. S. Ciontea, F. Antohe, and C. R. Usein, Volume: ‏ 24 Issue: ‏ 1Pages: ‏ 103-110Published: ‏mar 2016 Revista Romana de Medicina de Laborator 2016, IF: 0.325

S100A4 and ERBB2 as co-factors in pancreatic cancer, Nastase, A., Dima, Sa, Tica, V., Florea, R. Sorop, A. Ilie, V. Eftimie, M. Paslaru, L. Herlea, V. Bacalbasa, N. Ivan, L. Uyy, E. Suica, V.I. Antohe, F. Iancu, I.V. Plesa, A. Botezatu, A. Duda, D.G. Diaconu, C.C., Volume 21, Issue 4, December 2016, Pages 259-266 Journal of Translational Medicine and Research, 2016

Testosterone stimulates proliferation and preserves stemness of human adult mesenchymal stem cells and endothelial progenitor cells. Corotchi MC, Popa MA, Simionescu M. 2016;57(1):75-80. Rom J Morphol Embryol, 2016, IF:0.670

 

 2015

Alteration of actin dependent signaling pathways associated with membrane microdomains in hyperlipidemia, V. I. Şuică, E. Uyy, R. M. Boteanu, L. Ivan, and F. Antohe, Volume: ‏ 13Article Number: 30 Published: ‏ dec 1 2015 Proteome Science 2015 IF: 1.746

High-mobility group box 1 enhances the inflammatory process in diabetic lung, R. M. Boteanu, E. Uyy, V. I. Şuică, and F. Antohe, Volume: ‏ 583 Pages: ‏ 55-64 Published: ‏oct 1 2015 Archives of Biochemistry and Biophysics 2015 IF: 2.807

Mass spectrometry-based proteomics, Antohe, F., Volume: ‏ 11Issue: ‏ 2 Pages: ‏ 139-142 Published: apr-iun 2015 Acta Endocrinologica-Bucharest 2015 IF: 0.235

Carbapenemase-Producing Klebsiella pneumoniae in Romania: A Six-Month Survey, B. E. Lixandru, A. I. Cotar, M. Străuţ, C. R. Usein, D. Cristea, S. Ciontea, D. Tatu-Chiţoiu, I. Codiţă, A. Rafila, M. Nica, M. Buzea, A. Baicus, M. C. Ghiţă, I. Nistor, C. Tuchilus, M. Indreas, F. Antohe, C. Glasner, H. Grundmann, A. Jasir, and M. Damian Volume: ‏ 10Issue: ‏ 11Article Number: e0143214 Published: ‏ nov 23 2015 Plos One 2015 IF: 3.057

An in vitro method for adhesion of fresh adult murine heart slices on Collagen - Coated surfaces, Popa MA & Corotchi MC, Annals of the Romanian Society for Cell Biology 20 35-39. 2015.

Effects of 17- β estradiol in a novel co- culture system of human Wharton’s jelly-derived mesenchymal stem cells with adult- heart murine ventricular slices on cardiac regeneration. Corotchi MC* & Popa MA*, Atherosclerosis 241 e93. (*equal contribution), 2015, IF:3.942

 

 2014

Molecular characterization of staphylococcus aureus strains isolated from infective endocarditis, Oprea, M., Patriche DS, Străuţ M, Antohe F, Volume 73, Issue 3-4, 1 July 2014, Pages 74-83, Roumanian archives of microbiology and immunology 2014

 

 

National and International Grants (2014-2024)

2023–2026: PNRR-III-C9-2022–I8: CF 760061/23.05.023: Development of a new immunomodulatory therapy to reduce cardiac inflammation in myocardial infarction, myocarditis, and sepsis; https://www.icbp.ro/static/en/en-networking_grants-grants/pnrr.html

2022–2024: PN-III-P4-PCE-2021-1344, Contract number: PCE 114/2022: Molecular markers for prediction of evolution and prognosis in diabetic foot management, Acronym: DIAMARK; https://www.icbp.ro/static/en/en-networking_grants-grants-national_grants/diamark.html

2022-2024: PN-III-P1-1.1-TE-2021-1161: Circulating chaperone molecules in blood – potential predictors of gestational diabetes evolution; a proteomic analysis; https://www.icbp.ro/static/en/en-networking_grants-grants-national_grants/pregdm.html

2018-2022: PN-III-P4-ID-PCCF-2016-0172: Targeting innate immunity mechanisms for better risk stratification and identification of new therapeutic options in myocardial infarction, INNATE-IM; https://www.icbp.ro/static/en/en-networking_grants-grants-national_grants/innatemi.html

2018-2020: PNIII-P1-1.1-PD-2016-1369: Dynamics of endothelial junctional proteins in HMGB1-induced angiogenesis; Clinical implications. https://www.icbp.ro/static/en/en-networking_grants-grants-national_grants/epangio.html

2020-2021: PN-III-P1-1.1-PD2019-1234, Contract with No. PD160 / 2020, The role of the interaction between mesenchymal stromal cells and fibroblasts in the modulation of cardiac fibrosis by matrix metalloproteinase CD147; https://www.icbp.ro/static/en/en-networking_grants-grants-national_grants/fibrostem.html

2018-2021: XploreCAD; ERA-NET on Cardiovascular Diseases: Advanced ex vivo analyses and multi-frequency ultrasound technology for improved evaluation and diagnosis of coronary plaque (ERA-CVD Contract 41/2018); https://www.icbp.ro/static/en/en-networking_grants-grants-international/xplorecad.html

2015-2018: Norway Research Council - FRIPRO program: Multifunctional microbubbles for improved image-based diagnosis and drug delivery; https://www.sintef.no/en/projects/2015/multibubble/

2012-2016: PN-II-PCCA-2011-3: Fabrication of osteoinductive orthopedic implants with a hierarchical 3D gradient structure (FABIO3D); https://www.icbp.ro/static/en/en-networking_grants-grants-national_grants/fabio3d.html

2012-2016: PNII-PT-PCCA 2011-3, (CF 135/2012): Molecular markers as predictors of post-therapeutic evolution and prognosis in the management of differentiated thyroid carcinoma (GENITIR); https://www.icbp.ro/static/en/en-networking_grants-grants-national_grants/diamark.html

2012-2016: PN-II-PT-PCCA-2011-3.2-1490:The role of S100A4 and MAP4K4 in the progression of pancreatic ductal adenocarcinoma; S100MAP https://www.icbp.ro/static/en/en-networking_grants-grants-national_grants/s100map.html