Research
CELL CULTURE LABORATORY
Coordinator: Irina Titorencu, CS2, PhD
Role:
To establish and characterize original pure cell lines; cryo-preservation
Growing and cryo-preservation of imported cell lines
Development of new procedures for cell isolation, selection and culture
Training researchers in cell culture methods
Within the CARDIOPRO project the cell culture unit was radically remodelled, according to clean rooms technology and EU ISO 14644.1 regulations as follows:
- Compartment A1-for media preparation, storage and sterilization (ISO CLASS 8) and quarantine (ISO CLASS 7)
- Compartment A2 – for cell lines propagation and in vitro experiments (ISO CLASS 6)
- Compartment A3 – for special technologies intended for use in human regenerative medicine (ISO CLASS 5, GMP exigencies)
- The modernized cell culture laboratory is equipped with last generation 6 laminar flow hoods, 12 uni(tri) gas incubators, 5 inverted microscopes, centrifuges, cell counter, automatic washing machine.
The ICBP’s cell culture collection contains over 75 different cell lines, (original and/or expanded in the laboratory). Recently, a bank of human osteoprogenitor cell units, derived from bone morrow was started up for the use in bone pathology and traumatology as autotransplant.
The cell culture collection is currently utilized in numerous projects.
EXPERIMENTAL MODELING PLATFORM
Coordinator: Madalina Fenyo, Ph.D.; Marian Culcescu, Veterinary Doctor
This completely new facility provides SPF conditions for housing laboratory animals in accordance with the European standards. In addition the new equipments offer researchers’ modern facilities for imaging and non-invasive exploration, determination of patho-physiological parameters, transplantation studies, and rederivation/embryo transfer techniques.
CORE FACILITIES
Surgical rooms equipped for micro-, and macrosurgery
Electron microscope; Nikon fluorescence microscope
FPLC-HPLC system, Multiscan, Spectrofluorimeter and Image Master VDS
Ultracentrifuges with appropriate rotors
Thermal Cyclers PCR/RT-PCR
Real Time PCR
Gene Pulser
Small vessel wire Myograph
Nitric Oxide Measuring System
Each laboratory accommodates common equipment for chromatography, isoelectric focusing, electrophoresis, electrotransfer, UV-VIS spectrophotometry, and hoods.
CENTRAL SERVICES
Internet online connection and intranet PC network.
Library: journal subscriptions, journal collections, and about 1000 books.
OTHER FACILITIES
Aula “George E. Palade” (120 seats) equipped with audio visual facilities and IT support
OctoCell, NeoCell, PeriCell - seminars and communication rooms, journal club and poster display, and team building areas.
Cold rooms (3), stock rooms, maintenance and technical assistance.