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Grant of the Romanian National Authority for Scientific Research (CNCS – UEFISCDI)

PN-III-P1-1.1-PD-2016-1660, No. 19 ⁄ 2018 acronym BIOPRINT, Period: May 2, 2018 - April 30, 2020. Budget: 250.000 lei)



Project title:

Tissue engineering of blood vessels using three-dimensional bioprinting of endothelial and smooth muscle progenitor cells


Project team:

Dr. Florin Iordache, Project Director

Dr. Adriana Georgescu, Mentor



Project summary:

   The goal of tissue engineering is to generate living tissue constructs in vitro

that are morphologically and functionally similar to native tissue. Growing physiologically relevant

tissues requires multidisciplinary research where the resulting tissues can be used for the study of

human development and disease, to test the efficacy and toxicity of compounds and treatments,

and for regenerative medicine applications. Lately, the tissue engineering of a small-diameter

blood vessel substitute has become very important for cardiovascular researchers. In vascular

surgery blood vessel grafts made of synthetic polymers have the disadvantage of thrombosis,

rejection, intimal hyperplasia, calcification, chronic local inflammation. A new alternative may

come using tissue engineering of blood vessels that uses biodegradable 3D scaffolds as an

alternative for extracellular matrix, and stem cells that are seeded into the scaffolds. Thus, the

purpose of proposed project is to create artificial blood vessels using endothelial (EPCs) and

smooth muscle (SPCs) progenitor cells and polymeric biomaterials based hydrogels as scaffold.

Specifically, the efficiency of medical scaffolds and their influence on eukaryotic cells are the most

important parameters in determining the impact of these biomedical materials. Biocompatibility

and organization of progenitor cells will be evaluated at biochemical, cellular and molecular level.

The functionality of blood vessel construct will be evaluated using myograph techniques. The

elements of originality and innovation of the project consist in the experimental design that

combines the different progenitor cells with the new 3D technology that permit a better and

controlled deposition of cells in a 3D scaffold. With the proposed project, we aim to provide proof-

of-concept that tissue engineering of blood vessels using 3D bioprinting of EPCs and SPCs, might

represent a valuable new therapeutic strategy for cardiovascular regenerative medicine.



1. Obtaining and characterization of the progenitor cells and blood vessel structure/model.


2. Accomplishing 3D-printed blood vessel structure and investigating biocompatibility of EPCs and SPCs in the presence of BioInk hydrogel.


3. Evaluating morphology and adhesion ability of EPCs and SPCs embedded in BioInk hydrogel.


4. Structural and functional characterization of vascular structure obtained using 3D bioprinter.




Dissemination of results Stage III / 2020



1. Florin Iordache, Dorin Alexandru, Aurelia Magdalena Pisoschi, Aneta Pop. 3D Bioprinting of blood vessel model using collagen-hyaluronic acid hydrogel. AgroLife Scientific Journal, 8 (2), 1-5, 2019.

2. Ionela Andreea Neacsu, Adriana Petruta Serban, Adrian Ionut Nicoara, Roxana Trusca, Vladimir Lucian Ene, Florin Iordache. Biomimetic Composite Scaffold Based on Naturally Derived Biomaterials. Polymers, 2020, 12, 1161; doi:10.3390/polym12051161 (IF = 3.42).

3. Filis Curti, Izabela-Cristina Stancu, Georgeta Voicu, Horia Iovu, Cristina-Ioana Dobrita, Lucian Toma Ciocan, Rodica Marinescu, Florin Iordache. Development of 3D bioactive scaffolds through 3D 3 printing using wollastonite-gelatin inks. Polymers, 2020, (IF = 3.42).


Dissemination of results: Stage II / 2019


1. Razvan Airini*, Florin Iordache*, Dorin Alexandru, Lorand Savu, Florin Bogdan, Epureanu Dan Mihailescu, Bogdan Amuzescu, Horia Maniu. Senescenceinduced immunophenotype, gene expression and electrophysiology changes in human amniocytes. J Cell Mol Med. 2019 Nov;23(11):7233-7245. (IF= 4.65, *equal contribution).

2. Ioannis L. Liakos, Charlotte Menager, Nathanael Guigo, Alina Maria Holban, Florin Iordache, Francesca Pignatelli, Alexandru Mihai Grumezescu, Barbara Mazzolai, Nicolas Sbirrazzuoli. Suberin/trans-cinnamaldehyde oil nanoparticles with antimicrobial activity and anticancer properties when loaded with paclitaxel. ACS Appl. Bio Mater., 2019, 2, 83, 484-3497.

3. Andrei-Vlad Zanfir, Nicusor Nenu, Georgeta Voicu, Alina-Ioana Badanoiu, Cristina-Daniela Ghitulica, Florin Iordache. Modified calcium silicophosphate cements with improved properties. Materials Chemistry and Physics 238, 2019, 121965, 1-9. (IF = 2.78).



1. Florin Iordache, Aneta Pop, Dorin Alexandru, Horia Maniu. 3D bioprinting of blood vessel model using collagen-hyaluronic acid hydrogel. International Conference "Agriculture for Life, Life for Agriculture", Bucharest, Romania, 6-8 June 2019.

2. F. Iordache, D. Alexandru, A. Georgescu, H. Maniu. Differentiation of human amniotic stem cells into endothelial and muscle progenitor cells for blood vessels engineering. 44th FEBS Congress, Krakow, Poland, 6-11 July 2019

3. Florin Iordache, Dorin Alexandru, Horia Maniu. Engineering of Blood Vessel Using 3D Bioprinting of Stem Cells and Collagen-Hyaluronic Acid Hydrogel. 21st Romanian International Conference on Chemistry and Chemical Engineering, Constanta, Romania, 4-7 September 2019.





Dissemination of results: Stage I / 2018


1. F. Iordache, D. Alexandru, P. Gurban, H. Maniu. 3D bioprinting of human stem cells to construct blood vessel structures. The 43rd FEBS Congress “Biochemistry Forever”, 7-12 June 2018, Prague, Czech Republic.




2. F. Iordache, D. Alexandru, A. Georgescu, R. Airini, B. Amuzescu, L. Savu, H. Maniu. Characterization of senescent versus early passages human amniotic fluid stem cellsThe 12th Central and Eastern European Proteomic Conference, 24-26 October 2018, Bucharest. 




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 FINAL RAPORT FOR PROJECT PN-III-P1-1.1-PD-2016#1660, No. 19 ⁄ 2018 acronym BIOPRINT 


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